Molecular markers for self-compatibility in Japanese apricot (Prunus mume)
Molecular markers for self-compatibility in Japanese apricot (Prunus mume)
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DOI：
10.21273/hortsci.35.6.1121
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发表时间：
2000-10-01
期刊：
影响因子：
1.9
通讯作者：
Iwamoto, K
中科院分区：
文献类型：
--
作者：
Tao, R;Habu, T;Iwamoto, K
以梅（Prunus mume Sieb.等Zucc。）与自交不亲和的植物相比，具有园艺优势，因为不需要传粉者。自交不亲和性是配子体，在其他李属物种。本研究以其它李属植物的S-核糖核酸酶（S-RNases）为基础，寻找分子标记来鉴定自交亲和品种。根据李属S-RNases基因保守区设计寡核苷酸引物，对5个自交不亲和品种和6个自交亲和品种的总DNA进行PCR扩增。自交不亲和品种间有一条约1.5kbp的共同带。用编码甜樱桃（Prunus avium L.）S ′-RNase的cDNA探针对自交亲和品种的基因组DNA进行HindIII酶切，也显示出一条约12.1kbp的共同带。这些结果表明，日本杏自交亲和品种具有共同的S-RNase等位基因，可以作为自交亲和性的分子标记。
Self-compatible cultivars of Japanese apricot (Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no pollinizer is required. Self-incompatibility is gametophytic, as in other Prunus species. We searched for molecular markers to identify self-compatible cultivars based on the information about S-ribonucleases (S-RNases) of other Prunus species. Total DNA isolated from five self-incompatible and six self-compatible cultivars were PCR-amplified by oligonucleotide primers designed from conserved regions of Prunus S-RNases. Self-compatible cultivars exhibited a common band of approximate to1.5 kbp. Self-compatible cultivars also showed a common band of approximate to 12.1 kbp when genomic DNA digested with HindIII was probed with the cDNA encoding S'-RNase of sweet cherry (Prunus avium L.). These results suggest that self-compatible cultivars of Japanese apricot have a common S-RNase allele that can be used as a molecular marker for self-compatibility.