Na/K-ATPase activity and osmo-ionic regulation in adult whiteleg shrimp Litopenaeus vannamei exposed to low salinities
Na/K-ATPase activity and osmo-ionic regulation in adult whiteleg shrimp Litopenaeus vannamei exposed to low salinities
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DOI：
10.1016/j.aquaculture.2010.03.025
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发表时间：
2010-06-15
期刊：
影响因子：
4.5
通讯作者：
Wilder, Marcy
中科院分区：
文献类型：
--
作者：
Do Thi Thanh Huong;Jasmani, Safiah;Wilder, Marcy
在暴露于低盐度的凡纳滨对虾中检查了 Na/K-ATP 酶活性和渗透离子调节。暴露于极低盐度（0.5 ppt 和 1 ppt）的虾的血淋巴渗透压在 6 小时后迅速下降，从 800 mOsm 降至 540-560 mOsm；这些人无法存活超过 1 天。在暴露于 7 ppt 的情况下，渗透压水平分别在 6 小时和 1 天后迅速下降至 630 和 570 mOsm，而在暴露于 18 ppt 的虾中，渗透压水平在 6 小时后从 800 mOsm 缓慢下降至 700 mOsm，此后 7 天保持稳定，直到实验结束。血淋巴钠离子水平显示出与血淋巴渗透压相似的变化模式，初始水平为380 mmol/L，在极低盐度下降至180 mmol/L。还研究了钙、钾和镁离子浓度的变化。在实验期间，对照个体的鳃中Na/K-ATP酶活性为3.98μmol ADP/mg蛋白质/小时，维持在28ppt。暴露于 18 ppt、7 ppt 和 3 ppt 7 天的个体中，水平分别增加至 5.06、6.51 和 5.81 mumol ADP/mg 蛋白质/小时。综上所述，南美白对虾在低盐度下无法生存（
Na/K-ATPase activity and osmo-ionic regulation were examined in Litopenaeus vannamei exposed to low salinities. The hemolymph osmolality of shrimp exposed to very low salinities (0.5 ppt and 1 ppt) decreased rapidly after 6 h from 800 mOsm to 540-560 mOsm; these individuals could not survive beyond 1 day. Under exposure to 7 ppt, osmolality levels decreased rapidly to 630 and 570 mOsm after 6 h and 1 day respectively, whereas in shrimp exposed to 18 ppt, levels declined slowly from 800 mOsm to 700 mOsm after 6 h and thereafter remained stable for 7 days until the end of the experiment. Hemolymph sodium ion levels showed a pattern of change similar to that of hemolymph osmolality, with initial levels being 380 mmol/L and decreasing to 180 mmol/L under very low salinity. Changes in calcium, potassium, and magnesium ion concentrations were also investigated. Na/K-ATPase activity was 3.98 mu mol ADP/mg protein/hour in the gills of control individuals maintained at 28 ppt for the experimental duration. Levels increased in individuals exposed to 18 ppt, 7 ppt and 3 ppt for 7 days to 5.06, 6.51, and 5.81 mu mol ADP/mg protein/hour, respectively. Based on the above, it is suggested that L vannamei could not survive in low salinity (