XENOPUS CHORDIN - A NOVEL DORSALIZING FACTOR-ACTIVATED BY ORGANIZER-SPECIFIC HOMEOBOX GENES
XENOPUS CHORDIN - A NOVEL DORSALIZING FACTOR-ACTIVATED BY ORGANIZER-SPECIFIC HOMEOBOX GENES
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DOI：
10.1016/0092-8674(94)90068-x
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发表时间：
1994-12-02
期刊：
影响因子：
64.5
通讯作者：
DEROBERTIS, EM
中科院分区：
文献类型：
--
作者：
SASAI, Y;LU, B;DEROBERTIS, EM
通过差异筛选分离出非洲爪蟾基因，其表达可以被组织者特异性同源盒基因 goosecoid 和 Xnot2 激活。脊索蛋白基因编码一种由 941 个氨基酸组成的新型蛋白质，该蛋白质具有信号序列和四个富含 Cys 的结构域。弦蛋白的表达继鹅肠蛋白之后在斯佩曼组织中开始表达，并且激活素的诱导需要从头合成蛋白质。显微注射脊索蛋白 mRNA 诱导双轴，可以完全挽救腹侧胚胎的轴发育。该分子是一种有效的背侧化因子，在正确的时间和位置表达，以调节头部、躯干和尾部发育组织中心的细胞间相互作用。
A Xenopus gene whose expression can be activated by the organizer-specific homeobox genes goosecoid and Xnot2 was isolated by differential screening. The chordin gene encodes a novel protein of 941 amino acids that has a signal sequence and four Cys-rich domains. The expression of chordin starts in Spemann's organizer subsequent to that of goosecoid, and its induction by activin requires de novo protein synthesis. Microinjection of chordin mRNA induces twinned axes and can completely rescue axial development in ventralized embryos. This molecule is a potent dorsalizing factor that is expressed at the right time and in the right place to regulate cell-cell interactions in the organizing centers of head, trunk, and tail development.