Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy
Fingerprinting a Living Cell by Raman Integrated Mid-Infrared Photothermal Microscopy
复制标题
通过拉曼集成中红外光热显微镜对活细胞进行指纹识别
DOI：
10.1021/acs.analchem.9b02286
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发表时间：
2019-08-20
影响因子：
7.4
通讯作者：
Cheng, Ji-Xin
中科院分区：
文献类型：
--
作者：
Li, Xiaojie;Zhang, Delong;Cheng, Ji-Xin
基于红外吸收或拉曼散射的振动光谱成像技术可实现生物系统的非侵入性化学特异性可视化。具有不同选择规则的红外和拉曼模式提供了补充信息。具体来说，红外显微镜在指纹区域提供强信号，但空间分辨率较低。拉曼显微镜提供亚微米分辨率，但需要较长的采集时间。我们开发了一种系统，通过将共焦拉曼显微光谱学与最近开发的中红外光热显微镜相结合，结合了两种技术的优点。该混合系统能够以亚微米分辨率对活细胞进行快速红外光热成像，以识别感兴趣的点，然后对识别的物体进行全光谱拉曼分析。此外，还可以通过扫描红外激光的波长来获取指纹光热光谱。在脂肪细胞和单个细菌中展示的活细胞的全面振动指纹图谱有望将该技术在生物学和材料科学中广泛应用。
Vibrational spectroscopic imaging techniques, based on infrared absorption or Raman scattering, allow for noninvasive chemically specific visualization of biological systems. The infrared and Raman modalities with different selection rules provide complementary information. Specifically, infrared microscopy provides strong signals in the fingerprint region, but suffers from low spatial resolution. Raman microscopy provides submicrometer resolution, but requires a long acquisition time. We developed a system that combines the strengths of both techniques by integrating confocal Raman microspectroscopy to the recently developed mid-infrared photothermal microscopy. This hybrid system is capable of fast infrared photothermal imaging of living cells with submicrometer resolution to identify points of interest, followed by a full-spectrum Raman analysis of the identified objects. In addition, a fingerprint photothermal spectrum can be acquired by scanning the wavelengths of the infrared laser. Comprehensive vibrational fingerprint mapping of live cells, demonstrated in adipocytes and single bacteria, promises broad applications of this technology in biology and material science.