Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose
Structural identification of an polysaccharide isolated from Epimedium brevicornum and its beneficial effect on promoting osteogenesis in osteoblasts induced by high glucose
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DOI：
10.1016/j.biopha.2023.115893
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发表时间：
2023-11-17
影响因子：
7.5
通讯作者：
Li，Lin zi
中科院分区：
文献类型：
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作者：
Lei，Shan shan;Li，Bo;Li，Lin zi
目的糖尿病骨质疏松症（DOP）是一种由糖尿病引起的慢性骨代谢性疾病，其发病率不断上升。淫羊藿（Epimedium brevicornumMaxim， EB）是一种流行的中药，在中国用于治疗骨病已有数千年的历史。但其物质基础和具体作用机制尚不清楚。方法以短花楸粗多糖（EPE）为主要成分，检测从EPE中纯化的EBPC1的结构特征及其对高糖诱导的成骨细胞增殖、分化和细胞骨架的影响。结果EBPC1的分子量为10.5 kDa。主要由葡萄糖和半乳糖组成，EBPC1的主链为→4)-α- d - galp -(1→4)-α- d - galp -(1→6)-β- d - galp -(1→6)-β- d - galp -(1→4)-α- d - glcp -(1→4)-α- d - glcp -(1→4)-α- d - glcp -（1→4）。体外实验结果显示，EBPC1显著提高了原代成骨细胞碱性磷酸酶（ALP）活性和矿化结节形成，并在EBPC1预处理下显著上调了alpmrna和runx2mrna的表达。此外，EBPC1通过调控Bax/Bcl2调控细胞凋亡。结论EBPC1可促进成骨细胞成骨，其作用机制可能是通过调节Bax/Bcl2，促进成骨细胞成骨，从而改善成骨细胞凋亡。
AimDiabetes osteoporosis (DOP) is a chronic bone metabolic disease induced by diabetes, whose morbidity continues to increase.Epimedium brevicornumMaxim (EB), a popular Chinese traditional medicine, has been used to treat bone diseases in China for thousands of years. But its material basis and specific mechanism of action are not clear.MethodsEpimedium brevicornumcrude polysaccharide (EPE) is the main component, in this research the characterized the structure of EBPC1 purified from EPE was detected and its effects on cell proliferation, differentiation, and cytoskeletal in osteoblasts induced by high glucose.ResultsThe molecular weight of EBPC1 was 10.5 kDa. It was mainly comprised of glucose and galactose, and the backbone of EBPC1 was→4)-α-D-Galp-(1→4)-α-D-Galp-(1→6)-β-D-Galp-(1→6)-β-D-Galp-(1→4)-α-D-Glcp-(1→4)-α-D-Glcp-(1→. The results from in vitro experiments revealed that EBPC1 significantly increased alkaline phosphatase (ALP) activity and mineralized nodule formation in primary osteoblasts, also significantly up-regulated expression ofAlpmRNA andRunx2mRNA in the presence of EBPC1 pretreatment. Moreover, EBPC1 modulated apoptosis via the regulation of Bax/Bcl2.ConclusionThese results indicate that EBPC1 treatment can promote osteogenesis during DOP, which can ameliorate apoptosis by regulating Bax/Bcl2 and accelerating osteogenesis in osteoblasts.