Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor.
Structure and functional expression of the human macrophage inflammatory protein 1 alpha/RANTES receptor.
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DOI：
10.1084/jem.177.5.1421
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发表时间：
1993-05-01
影响因子：
15.3
通讯作者：
Murphy, P M
中科院分区：
文献类型：
--
作者：
Gao, J L;Kuhns, D B;Tiffany, H L;McDermott, D;Li, X;Francke, U;Murphy, P M
趋化因子β家族由至少六种不同的细胞因子组成，其调节哺乳动物物种中吞噬细胞和淋巴细胞的运输;其中至少一种巨噬细胞炎性蛋白1 α（MIP-1 α）也调节造血干细胞的生长。我们现在表明，MIP-1 α和相关的β趋化因子，RANTES，诱导细胞内Ca 2+浓度在多形核白细胞，可以被抑制和特异性脱敏，提示一个共同的受体的瞬时变化。此外，我们现在已经克隆了该受体的cDNA和基因，在非洲爪蟾卵母细胞中功能性表达该受体，并将该基因定位于人类染色体3 p21。在成熟和未成熟的骨髓细胞以及B细胞中发现了该受体的转录物。该受体是G蛋白偶联受体超家族的成员。它与α趋化因子白细胞介素8的受体具有约33%的氨基酸同一性，并且可能是US 28产物的人同源物，US 28是人巨细胞病毒的开放阅读框架。
The chemokine beta family is comprised of at least six distinct cytokines that regulate trafficking of phagocytes and lymphocytes in mammalian species; at least one of these, macrophage inflammatory protein 1 alpha (MIP-1 alpha), also regulates the growth of hematopoietic stem cells. We now show that MIP-1 alpha and the related beta chemokine, RANTES, induce transient alterations in intracellular Ca2+ concentration in polymorphonuclear leukocytes that can be reciprocally and specifically desensitized, suggesting a common receptor. Moreover, we have now cloned both the cDNA and the gene for this receptor, functionally expressed the receptor in Xenopus oocytes, and mapped the gene to human chromosome 3p21. Transcripts for the receptor were found in mature and immature myeloid cells as well as B cells. The receptor is a member of the G protein-coupled receptor superfamily. It has approximately 33% amino acid identity with receptors for the alpha chemokine, interleukin 8, and may be the human homologue of the product of US28, an open reading frame of human cytomegalovirus.