Association of distinct yeast Not2 functional domains with components of Gcn5 histone acetylase and Ccr4 transcriptional regulatory complexes
Association of distinct yeast Not2 functional domains with components of Gcn5 histone acetylase and Ccr4 transcriptional regulatory complexes
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DOI：
10.1093/emboj/17.22.6714
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发表时间：
1998-11-16
期刊：
影响因子：
11.4
通讯作者：
Struhl, K
中科院分区：
文献类型：
--
作者：
Benson, JD;Benson, M;Struhl, K
NOT基因最初是在酵母遗传筛查中发现的，该筛查选择了导致HIS3启动子非共识T-C TATA元件利用增加的突变。在这里，我们提出了Notz的N-末端与Ada/Gcn5组蛋白乙酰转移酶复合体的成分相互作用的证据。由于Not2 N末端功能的丧失或ada2或gcn5的缺失，这种相互作用的丧失会导致HIS3Te元件的去抑制。这表明Not2与Ada/Gcn5组蛋白乙酰转移酶复合体的结合参与了HIS3启动子的调控。最近也观察到NOT和CCR4转录调控复合体之间的关联。我们的表型分析表明，这些与CCR4相关的Not2功能是由Not2的一个功能独立结构域介导的，该结构域包括高度保守的C-末端，即含有酵母Not2 N-末端的嵌合蛋白，在酵母中融合到人的C-末端功能，这表明Not2 C-末端代表了一个独特的模块结构域，其功能在高等和低等真核生物之间是保守的。
The NOT genes were originally identified in a yeast genetic screen that selected mutations resulting in increased utilization of a non-consensus T-C TATA element of the HIS3 promoter. Here, we present evidence that the N-terminus of NotZ interacts with components of the Ada/Gcn5 histone acetyltransferase complex. Loss of this interaction either through abrogation of Not2 N-terminal function or deletion of ada2 or gcn5 results in derepression of the HIS3 Te element. This suggests that association of Not2,vith the Ada/Gcn5 histone acetyltransferase complex is involved in regulation of the HIS3 promoter. Association between the Not and CCR4 transcriptional regulatory complexes has also been observed recently. Our phenotypic analyses suggest that these CCR4-related Not2 functions are mediated by a functionally independent domain of Not2 that includes the highly conserved C-terminus, Chimeric proteins containing the yeast Not2 N-terminus fused to the human C-terminus function in yeast, suggesting that the Not2 C-terminus represents a distinct modular domain whose function is conserved between higher and lower eukaryotes.