Sequence analysis for the complete proviral genome of subgroup J Avian Leukosis virus associated with hemangioma: a special 11 bp deletion was observed in U3 region of 3'UTR.
Sequence analysis for the complete proviral genome of subgroup J Avian Leukosis virus associated with hemangioma: a special 11 bp deletion was observed in U3 region of 3'UTR.
复制标题
DOI：
10.1186/1743-422x-8-158
复制
发表时间：
2011-04-08
期刊：
影响因子：
4.8
通讯作者：
Huang Y
中科院分区：
文献类型：
--
作者：
Shi M;Tian M;Liu C;Zhao Y;Lin Y;Zou N;Liu P;Huang Y
J亚群禽白血病病毒（ALV-J）属于逆转录病毒，可引起家禽和野禽的肿瘤。骨髓细胞瘤病是感染鸡群中最常见的肿瘤，但近年来由ALV-J引起的血管瘤病例报道较少。分离到一株与血管瘤相关的ALV-J株SCDY 1，并测定了其前病毒基因组序列。SCDY 1基因全长7489 nt。SCDY 1与GenBank中其它毒株env、pol和gag基因核苷酸同源性分别为90.3- 94.2%、96.6- 97.6%和94.3-96.5%，氨基酸同源性分别为85.1- 90.7%、97.4- 98.7%和96.2-98.4%。以HPRS-103为参照序列对ALV-J株基因组序列进行比对分析，结果表明，SCDY 1株和另一株ALV-J血管瘤分离株NHH株在3 'UTR的U3区有一个11bp的特异性缺失，SCDY 1株基因组中不存在非功能性的TM和E元件，而转录调控元件C/EBP、E2 BP、NFAP-1、CArG box和Y box高度保守。系统发育分析表明，所有ALV-J株可分为4个类群，SCDY 1和另一株NHH属于同一类群。包膜糖蛋白基因的变异程度高于其他基因。SCDY 1基因组序列与另一株从血管瘤中分离的ALV-J株NHH基因组序列有较近的同源性。从血管瘤病例中分离到的ALV-J基因组在3 'UTR的U3区有一个11bp的缺失，这可能与血管瘤的发生有关。
Avian Leukosis virus (ALV) of subgroup J (ALV-J) belong to retroviruses, which could induce tumors in domestic and wild birds. Myelocytomatosis was the most common neoplasma observed in infected flocks; however, few cases of hemangioma caused by ALV-J were reported in recent year. An ALV-J strain SCDY1 associated with hemangioma was isolated and its proviral genomic sequences were determined. The full proviral sequence of SCDY1 was 7489 nt long. Homology analysis of the env, pol and gag gene between SCDY1 and other strains in GenBank were 90.3-94.2%, 96.6-97.6%, and 94.3-96.5% at nucleotide level, respectively; while 85.1-90.7%, 97.4-98.7%, and 96.2-98.4% at amino acid level, respectively. Alignment analysis of the genomic sequence of ALV-J strains by using HPRS-103 as reference showed that a special 11 bp deletion was observed in U3 region of 3'UTR of SCDY1 and another ALV-J strain NHH isolated from case of hemangioma, and the non-functional TM and E element were absent in the genome of SCDY1, but the transcriptional regulatory elements including C/EBP, E2BP, NFAP-1, CArG box and Y box were highly conserved. Phylogenetic analysis revealed that all analyzed ALV-J strains could be separated into four groups, and SCDY1 as well as another strain NHH were included in the same cluster. The variation in envelope glycoprotein was higher than other genes. The genome sequence of SCDY1 has a close relationship with that of another ALV-J strain NHH isolated from case of hemangioma. A 11 bp deletion observed in U3 region of 3'UTR of genome of ALV-J isolated from case of hemangioma is interesting, which may be associated with the occurrence of hemangioma.