Improvement of arachidonic acid production by mutants with lower n-3 desaturation activity derived from Mortierella alpina 1S-4
Improvement of arachidonic acid production by mutants with lower n-3 desaturation activity derived from Mortierella alpina 1S-4
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DOI：
10.1007/s00253-004-1682-7
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发表时间：
2004-08
影响因子：
5
通讯作者：
E. Sakuradani;Y. Hirano;N. Kamada;M. Nojiri;J. Ogawa;S. Shimizu
中科院分区：
文献类型：
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作者：
E. Sakuradani;Y. Hirano;N. Kamada;M. Nojiri;J. Ogawa;S. Shimizu
从高山被孢霉（Mortierella alpina 1 S-4）中筛选到5株花生四烯酸（AA）高产突变株Y11、Y135、Y164、Y180和Y 61。这被认为是由于n-3去饱和与AA转化为二十碳五烯酸的低活性或无活性，其在低于20°C的培养温度下起作用。在20°C高浓度葡萄糖条件下，Y11和Y 61的AA积累量分别为4.97 mg/ml和4.11 mg/ml，而M的AA积累量为3.74 mg/ml。阿尔卑斯山1 S-4。在5L发酵罐中，随着培养基中葡萄糖的消耗，Y11和Y 61的AA含量不断增加，第10天分别达到1.48 mg/ml和1.77 mg/ml（118 mg/g和120 mg/g干菌丝），而M的AA含量为0.95 mg/ml（86 mg/g干菌丝）。阿尔卑斯山1 S-4。从脂类分析结果来看，Y11和Y 61的主要脂类中TG含量显著高于M。Y11和Y 61的TG中AA含量也较高。Y11和Y 61都是富含AA的TG的潜在生产者。
Five mutants were obtained, Y11, Y135, Y164, Y180 and Y61, capable of accumulating higher amounts of arachidonic acid (AA) thanMortierella alpina1S-4, an industrial strain for the production of AA-rich triacylglycerol (TG). This is thought to be due to low or no activity of n-3 desaturation with conversion of AA to eicosapentaenoic acid, which functions at a cultural temperature below 20°C. In small-scale cultivation under optimum conditions, Y11 and Y61 respectively accumulated 4.97 mg/ml and 4.11 mg/ml of AA, using a high concentration of glucose at 20°C, compared with 3.74 mg/ml forM. alpina1S-4. In a 5-l jar fermentor, the AA content in Y11 and Y61 kept increasing during cultivation, with consumption of the glucose in the medium; and this reached 1.48 mg/ml and 1.77 mg/ml (118 mg/g, 120 mg/g of dry mycelia) at day 10, respectively, compared with 0.95 mg/ml (86 mg/g of dry mycelia) forM. alpina1S-4. From the results of lipid analysis, the TG contents of Y11 and Y61 in the major lipids were significantly higher than that ofM. alpina1S-4; and the AA percentages in TG of Y11 and Y61 were also higher. Both Y11 and Y61 are potential producers of TG rich in AA.