Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants.
Biosynthesis and secretion of several enzymes in Escherichia coli dnaK and dnaJ mutants.
复制标题
大肠杆菌 dnaK 和 dnaJ 突变体中几种酶的生物合成和分泌。
DOI：
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发表时间：
2000
期刊：
影响因子：
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通讯作者：
A. Jóźwik
中科院分区：
文献类型：
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作者：
K. Wolska;B. Lobacz;D. Jurkiewicz;E. Bugajska;M. Kuć;A. Jóźwik
Escherichia coli null dnaJ and dnaKdnaJ mutants were defective in the biosynthesis and secretion of several enzymes. The synthesis of beta-galactosidase induced in delta dnaJ and delta dnaKdnaJ mutants was abolished at 42 degrees C and significantly decreased at 30 and 37 degrees C. The activity of alkaline phosphatase in the periplasm in both mutant strains at high temperature was lower than in the wild-type strain. The synthesis of b-type cytochromes was defective in two deletion mutants while the synthesis of nitrate reductase-A at 42 degrees C was influenced by dnaK mutation only. The lack of DnaK and DnaJ does not impair the activity of catechol 2,3-dioxygenase irrespective of growth temperature.
