Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres
Intervertebral disc regeneration using platelet-rich plasma and biodegradable gelatin hydrogel microspheres
复制标题
DOI：
10.1089/ten.2006.0042
复制
发表时间：
2007-01-01
影响因子：
--
通讯作者：
Kubo, Toshikazu
中科院分区：
文献类型：
--
作者：
Nagae, Masateru;Ikeda, Takumi;Kubo, Toshikazu
本研究评价富含血小板血浆(PRP)对退变椎间盘(IVD)的体内再生作用。在诱导兔IVD变性后，我们用这些兔的血液离心法制备了PRP。将这些PRP注射到变性IVD的髓核(NP)中，然后将其植入明胶水凝胶微球中，明胶水凝胶微球可以物理化学的方式固定PRP生长因子，并随着微球的降解而持续释放它们。作为对照，注入磷酸盐缓冲盐水(PBS)的微球和不含微球的PRP。组织学上，在PBS对照组、单纯PRP组和Sham组，观察到IVD随时间推移的显著进展。相比之下，在PRP组的8周时间里，进展明显受到抑制。免疫组织化学显示，注射PRP微球8周后，纤维环内层和NP区蛋白多糖免疫组织化学染色明显增强。注射8周后，几乎所有的微球都是模糊的，在本研究中没有明显的副作用。我们的结果表明，PRP和明胶水凝胶微球联合注入IVD可能是治疗IVD退变的一种有前途的治疗方式。
This study evaluated the regenerative effects of platelet-rich plasma (PRP) for the degenerated intervertebral disc (IVD) in vivo. After induction of IVD degeneration in rabbits, we prepared PRP by centrifuging blood obtained from these rabbits. These PRP were injected into the nucleus pulposus (NP) of the degenerated IVDs after impregnation into gelatin hydrogel microspheres that can immobilize PRP growth factors physiochemically and release them in a sustained manner with the degradation of the microspheres. As controls, microspheres impregnated with phosphate-buffered saline (PBS) and PRP without microspheres were similarly injected. Histologically, notable progress in IVD degeneration with time courses was observed in the PBS control, PRP-only, and sham groups. In contrast, progress was remarkably suppressed over the 8-week period in the PRP group. Moreover, in immunohistochemistry, intense immunostaining for proteoglycan in the NP and inner layer of the annulus fibrosus was observed 8 weeks after administration of PRP-impregnated microspheres. Almost all microspheres were indistinct 8 weeks after the injection, and there were no apparent side effects in this study. Our results suggest that the combined administration of PRP and gelatin hydrogel microspheres into the IVD may be a promising therapeutic modality for IVD degeneration.