Xenopus Smad3 is specifically expressed in the chordoneural hinge, notochord and in the endocardium of the developing heart
Xenopus Smad3 is specifically expressed in the chordoneural hinge, notochord and in the endocardium of the developing heart
复制标题
DOI：
10.1016/s0925-4773(01)00365-3
复制
发表时间：
2001-06-01
影响因子：
2.6
通讯作者：
Hill, CS
中科院分区：
文献类型：
--
作者：
Howell, M;Mohun, TJ;Hill, CS
Smads是细胞内的信号分子，将信号从转化生长因子-β超家族成员的受体传递到细胞核。我们克隆了Smad3(XSmad3)的非洲爪哇同源基因。该基因与人Smad3基因在氨基酸水平上的同源性为94.6%。它被表达为母体的mRNA，在10.5期之后消失，但在尾芽早期重新出现。在早期发育阶段，它的含量远低于XSmad2。从第27阶段开始，XSmad3在整个头部区域和闪族区域用XSmad2表达。然而，令人惊讶的是，XSmad3单独在脊神经铰链、脊索和发育中的心脏中特异表达。进一步分析发现，XSmad3在心内膜中特异表达，但在心肌或心包中不表达。脊神经铰链染色至少持续到阶段40，而心内膜染色在大约阶段32/33达到高峰。(C)2001爱思唯尔科学爱尔兰有限公司，保留所有权利。
The Smads are intracellular signalling molecules that transduce signals from receptors for members of the TGF-P superfamily to the nucleus. We have cloned the Xenopus orthologue of Smad3 (XSmad3). It is 94.6% identical to human Smad3 at the amino acid level. It is expressed as a maternal mRNA which disappears after stage 10.5, but reappears at the early tailbud stages. It is much less abundant than XSmad2 at the early developmental stages. From Stage 27 onwards XSmad3 is expressed with XSmad2 throughout the head region and in the semitic region. Strikingly however, XSmad3 alone is specifically expressed in the chordoneural hinge, the notochord and in the developing heart. Closer analysis reveals that XSmad3 is specifically expressed in the endocardium but not in the myocardium or pericardium. The chordoneural hinge staining persists at least until stage 40 whereas the staining in the endocardium peaks at approximately stage 32/33. (C) 2001 Elsevier Science Ireland Ltd, All rights reserved.