Th2 differentiation is unaffected by Jagged2 expression on dendritic cells
Th2 differentiation is unaffected by Jagged2 expression on dendritic cells
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DOI：
10.4049/jimmunol.180.12.7931
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发表时间：
2008-06-15
影响因子：
4.4
通讯作者：
Pearce, Edward J.
中科院分区：
文献类型：
--
作者：
Krawczyk, Connie M.;Sun, Jie;Pearce, Edward J.
树突状细胞 (DC) 表达锯齿形缺口配体已被认为在指导 Th2 反应中发挥作用。支持这一假设的是，我们发现 Jagged2 而不是 Jagged1 的表达与 DC 诱导 Th2 反应的能力相关。 Jagged2 表达因蠕虫可溶性曼氏血吸虫卵 Ag 的反应而上调，该抗原调节 DC 诱导 Th2 反应，而在接触通常促进 Th1 反应的 TLR 激动剂后，Jagged2 表达显着下调。相反，TLR 连接显着诱导 Jagged1 表达。尽管存在这些相关性，但使用逆转录病毒递送的小干扰RNA抑制Jagged2的表达并不能影响DC在体外或体内诱导Th2细胞分化的能力。此外，逆转录病毒诱导的Jagged2表达并没有增强DC诱导Th2细胞反应的能力。我们的数据表明 DC 的 Jagged2 表达对于 Th2 细胞分化来说是不够的或必需的。
Expression of the Jagged Notch ligands by dendritic cells (DCs) has been suggested to play a role in instructing Th2 responses. Supporting this hypothesis, we found that Jagged2 but not Jagged1 expression, correlates with the ability of DCs to induce Th2 responses. Jagged2 expression is up-regulated in response to the helminth soluble Schistosoma mansoni egg Ag, which conditions DCs to induce Th2 responses, and is markedly down-regulated following exposure to TLR agonists that generally promote Th1 responses. Conversely, Jagged1 expression is markedly induced by TLR ligation. Despite these correlations, suppression of expression of Jagged2 using retrovirally delivered small interfering RNA failed to affect the ability of DCs to induce Th2 cell differentiation either in vitro or in vivo. Moreover, retrovirally induced expression of Jagged2 did not enhance the ability of DCs to induce Th2 cell responses. Our data indicate that Jagged2 expression by DCs is not sufficient or required for Th2 cell differentiation.