Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato
Elicitin recognition confers enhanced resistance to Phytophthora infestans in potato
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DOI：
10.1038/nplants.2015.34
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发表时间：
2015-03-30
期刊：
影响因子：
18
通讯作者：
Vleeshouwers, Vivianne G. A. A.
中科院分区：
文献类型：
--
作者：
Du, Juan;Verzaux, Estelle;Vleeshouwers, Vivianne G. A. A.
马铃薯晚疫病，由破坏性的爱尔兰饥荒病原体致病疫霉引起，是对全球粮食安全的主要威胁（1，2）。迄今为止鉴定的所有晚疫病抗性基因都属于卷曲螺旋、核苷酸结合、富含亮氨酸的重复细胞内免疫受体类（3）。然而，病原体的毒性小种迅速进化以逃避这些细胞质免疫受体的识别（4）。在这里，我们证明了受体样蛋白ELR（elicitin反应）从野生马铃薯Solanum microdontum介导的elicitin结构域，在疫霉属物种是保守的分子模式的细胞外识别。ELR与免疫辅助受体BAK 1/SERK 3相关联，并介导来自几种疫霉属物种的激发素蛋白的广谱识别，包括来自致病疫霉的四种不同的激发素。将ELR转入栽培马铃薯，可增强马铃薯对晚疫病菌的抗性。聚合细胞表面模式识别受体与细胞内免疫受体可以最大限度地提高对这种毁灭性植物病原体产生更广泛和更持久抗性的潜力。
Potato late blight, caused by the destructive Irish famine pathogen Phytophthora infestans, is a major threat to global food security(1,2). All late blight resistance genes identified to date belong to the coiled-coil, nucleotide-binding, leucine-rich repeat class of intracellular immune receptors(3). However, virulent races of the pathogen quickly evolved to evade recognition by these cytoplasmic immune receptors(4). Here we demonstrate that the receptor-like protein ELR (elicitin response) from the wild potato Solanum microdontum mediates extracellular recognition of the elicitin domain, a molecular pattern that is conserved in Phytophthora species. ELR associates with the immune co-receptor BAK1/SERK3 and mediates broad-spectrum recognition of elicitin proteins from several Phytophthora species, including four diverse elicitins from P. infestans. Transfer of ELR into cultivated potato resulted in enhanced resistance to P. infestans. Pyramiding cell surface pattern recognition receptors with intracellular immune receptors could maximize the potential of generating a broader and potentially more durable resistance to this devastating plant pathogen.