Respiratory rate and atp content of stria vascularis of guinea pig in vitro
Respiratory rate and atp content of stria vascularis of guinea pig in vitro
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豚鼠体外呼吸频率及血管纹atp含量
DOI：
10.1288/00005537-197811000-00011
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发表时间：
1978
期刊：
影响因子：
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通讯作者：
N. Marcus
中科院分区：
文献类型：
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作者：
D. C. Marcus;R. Thalmann;N. Marcus
体外培养豚鼠耳蜗血管纹，以确定其代谢反应的底物和培养介质的离子组成的变化。在含有葡萄糖和丙酮酸盐作为底物的培养基中，37°下的呼吸速率为17.3 ± 1.33（SEM，n = 51）μI O2/mg干重-小时。纹不能维持恒定的呼吸，仅仅依靠内源性燃料储备。在有底物供应的情况下，ATP水平可维持在体内水平的73%左右。葡萄糖似乎是一个足够的底物纹在体外，因为谷氨酸，丙酮酸，富马酸并没有增加呼吸频率。琥珀酸显著增加呼吸，但不增加ATP水平。哇巴因（10 - 4 M）导致呼吸频率下降48%。在无Na+和无K+培养基中孵育，均导致呼吸速率不可逆降低，与哇巴因引起的呼吸速率不可逆降低相当（或更大）。这些数据与纹状体中Na+-K+-ATP酶的高活性和内淋巴电位对哇巴因的显著敏感性雅阁。
Stria vascularis from guinea pig cochleae was incubated in vitro to determine its metabolic response to variations in substrate and ion composition of the incubation medium. The respiratory rate at 37° in a medium containing glucose and pyruvate as substrate was 17.3 ± 1.33 (SEM, n = 51) μI O2/mg dry weight‐hour. The stria could not maintain constant respiration by relying solely upon endogenous fuel stores. With substrate supplied, the ATP level could be maintained at about 73% of that existing in vivo. Glucose appears to be an adequate substrate for stria in vitro since glutamate, pyruvate, and fumarate did not increase the respiratory rate. Succinate increased respiration markedly but did not increase the ATP level. Ouabain (10−4 M) caused a 48% decrease in the respiratory rate. Incubation in Na+‐free and K+‐free medium, each resulted in irreversible decrease of respiratory rate comparable to (or greater than) that caused by ouabain. These data are in accord with the high activity of Na+‐K+‐ATPase in the stria and the pronounced sensitivity of the endolymphatic potential to ouabain.