Microtubule-dependent reticulopodial motility: is there a role for actin?
Microtubule-dependent reticulopodial motility: is there a role for actin?
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微管依赖性网状足运动：肌动蛋白有作用吗？
DOI：
10.1002/cm.970060212
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发表时间：
1986
影响因子：
--
通讯作者：
Bowser,SS
中科院分区：
文献类型：
--
作者：
Travis,JL;Bowser,SS
我们总结了我们最近对两种异形有孔虫网状有孔虫网状细胞骨架的免疫细胞化学特征以及我们对其运动性的药理学剖析。用脑微管相关蛋白2抗血清染色网足微管细胞骨架。罗丹明-鬼笔藻素染色显示聚合肌动蛋白定位于网足。微管抑制剂可逆地抑制运动的所有方面；细胞松弛素导致运动的形态改变和解体，但不抑制伪足运动或细胞内运输。同时应用KCN和水杨基异羟肟酸(一种替代的氧化酶抑制剂)迅速阻止了所有的运动，表明运动依赖于代谢能量，另一条氧化途径在异形藻中起作用。显微操作和激光显微外科实验显示网状足底各处都有张力。我们的结果表明，微管是网状足底运动机械的活跃组成部分。肌动蛋白可能介导底物黏附、全细胞运动、伪足张力和基于微管的运动的协调。
We summarize our recent immunocytochemical characterization of the reticulopodial cytoskeleton of two allogromiid foraminifers and our pharmacologic dissection of its motility. The reticulopodial microtubule cytoskeleton stained with an antiserum to brain microtubule‐associated protein 2. Polymeric actin was localized in the reticulopodia by rhodamine‐phalloidin staining. Microtubule inhibitors reversibly inhibited all aspects of motility; cytochalasins induced altered morphology and disorganization of motility but did not inhibit pseudopodial movements or intracellular transport. Simultaneous application of KCN and salicylhydroxamic acid (an alternative oxidase inhibitor) rapidly blocked all movement, indicating that motility is dependent on metabolic energy and that an alternative oxidative pathway functions in allogromiids. Micromanipulation and laser microsurgical experiments revealed tension throughout the reticulopodium. Our results suggest that microtubules are active components of the reticulopodial motile machinery. Actin may mediate substrate adhesion, whole‐cell locomotion, pseudopodial tension, and coordination of the microtubule‐based motility.