Infrared up-converting phosphors for bioassays
Infrared up-converting phosphors for bioassays
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DOI：
10.1049/ip-nbt:20045014
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发表时间：
2005-04-05
期刊：
影响因子：
--
通讯作者：
Tanke, H. J.
中科院分区：
文献类型：
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作者：
Corstjens, P. L. A. M.;Li, S.;Tanke, H. J.
上转换磷光体报告物颗粒的发展为现代检测技术添加了强有力的工具。精心构建的磷光体报告分子具有核-壳结构，其具有适合于标准生物缀合的表面官能团。这些报告分子是化学稳定的，具有独特的红外上转换特性，并且易于检测。与传统的荧光报告物相比，上转换磷光体颗粒不会漂白，并且允许永久激发，同时进行信号积分。一个大的反斯托克斯位移（高达500 nm）分离离散的发射峰从红外激发源。沿着由于在红外激发时不存在自发荧光而导致的生物样本中的无与伦比的对比度，上转换磷光体技术（UPT）具有用于高灵敏度基于颗粒的测定的独特性质。本文综述了UPT报告颗粒的制备、特性及其在各种生物检测中的应用。
The development of up-converting phosphor reporter particles has added a powerful tool to modern detection technologies. Carefully constructed phosphor reporters have core-shell structures with surface functional groups suitable for standard bio-conjugations. These reporters are chemically stable, possess the unique property of infrared up-conversion, and are readily detected. In contrast to conventional fluorescent reporters, up-converting phosphor particles do not bleach and allow permanent excitation with simultaneous signal integration. A large anti-Stokes shift (up to 500 nm) separates discrete emission peaks from the infrared excitation source. Along with the unmatched contrast in biological specimens due to the absence of autofluorescence upon infrared excitation, up-converting phosphor technology (UPT) has unique properties for highly-sensitive particle-based assays. The production and characteristics of UPT reporter particles as well as their application in various bioassays is reviewed.