Anti-peptide antibodies to the P4502D subfamily in rat, dog and man.
Anti-peptide antibodies to the P4502D subfamily in rat, dog and man.
复制标题
大鼠、狗和人体内 P4502D 亚家族的抗肽抗体。
DOI：
10.3109/00498259509061910
复制
发表时间：
1995
期刊：
影响因子：
--
通讯作者：
S. Fujita
中科院分区：
文献类型：
--
作者：
A. Nakamura;Y. Yamamoto;T. Tasaki;C. Sugimoto;M. Masuda;A. Kazusaka;S. Fujita
1.为了获得P4502 D亚家族的特异性抗体，我们产生了针对合成肽DPAQPPRD（肽A）和DPTQPPRH（肽B）的两种抗肽抗体。肽A的序列存在于大鼠P4502 D2、P4502 D4和人P4502 D 6中，而肽B的序列存在于狗P4502 D亚家族中。这些序列与肝/肾微粒体自身免疫性肝炎的表位密切相关。2.在免疫印迹研究中，抗肽A的抗肽抗体识别来自表达P4502 D 6的人淋巴母细胞和大鼠肝脏的微粒体中的49-KDa蛋白。与狗肝微粒体无交叉反应。相反，抗肽B的抗肽抗体仅在狗肝微粒体中识别49-KDa蛋白。这些表明，每种抗肽抗体对P4502 D亚家族成员的相应序列具有特异性，具有本文研究的物种。3.在免疫抑制研究中，针对肽B的抗肽抗体抑制由狗P4502 D亚家族介导的丁硝基洛尔4-羟基化和普萘洛尔4，5-羟基化。这些数据表明，针对肽B的抗肽抗体结合P4502 D亚家族的天然和变性形式。4.本研究表明，针对该区域的抗肽抗体对于研究P4502 D亚家族成员是有用的。
1. In order to obtain specific antibodies of the P4502D subfamily, we generated two anti-peptide antibodies against synthetic peptides, DPAQPPRD (peptide A) and DPTQPPRH (peptide B). The sequence of peptide A occurs in rat P4502D2, P4502D4 and human P4502D6, whereas the sequence of peptide B occurs in the dog P4502D subfamily. These sequences are closely related to an epitope of liver/kidney microsomal autoimmune hepatitis. 2. In immunoblotting studies, the anti-peptide antibody against peptide A recognized a 49-KDa protein in microsomes derived from human lymphoblasts expressing P4502D6 and rat liver. It showed no crossreactivity with microsomes from dog liver. In contrast, the anti-peptide antibody against peptide B recognized a 49-KDa protein only in microsomes of dog liver. These indicate that each anti-peptide antibody has the specificity for the respective sequences of the members of P4502D subfamily, with the species investigated herein. 3. In immunoinhibition studies, the anti-peptide antibodies against peptide B inhibited bunitrolol 4-hydroxylation and propranolol 4,5-hydroxylation, which are mediated by the dog P4502D subfamily. These data suggest that the anti-peptide antibodies against peptide B bind to the native and denatured forms of the P4502D subfamily. 4. The present study has demonstrated that the anti-peptide antibodies against this region are useful for studying the members of the P4502D subfamily.
DOI：
--
发表时间：
1994-07
期刊：
The Journal of pharmacology and experimental therapeutics
影响因子：
--
作者：
T. Shimada;H. Yamazaki;M. Mimura;Y. Inui;F. Guengerich
通讯作者：
T. Shimada;H. Yamazaki;M. Mimura;Y. Inui;F. Guengerich
影响因子：
15.9
作者：
MANNS, MP;GRIFFIN, KJ;JOHNSON, EF
通讯作者：
JOHNSON, EF