Structural organization and nucleotide sequence of mouse c-myb oncogene: activation in ABPL tumors is due to viral integration in an intron which results in the deletion of the 5' coding sequences
Structural organization and nucleotide sequence of mouse c-myb oncogene: activation in ABPL tumors is due to viral integration in an intron which results in the deletion of the 5' coding sequences
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小鼠 c-myb 癌基因的结构组织和核苷酸序列：ABPL 肿瘤中的激活是由于病毒整合在内含子中，导致 5 编码序列缺失
DOI：
10.1093/nar/14.13.5309
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发表时间：
1986
影响因子：
14.9
通讯作者：
E.Premkumar Reddy
中科院分区：
文献类型：
--
作者：
Sukadev Lavu;E.Premkumar Reddy
筛选小鼠DNA噬菌体文库中与v-myb癌基因同源的序列，分离出含有v-myb相关区域的两个重叠克隆。限制性内切酶图谱、异源双链分析和核苷酸序列分析显示存在9个外显子。这些外显子中的六个与v-myb区同源，而其他三个外显子来自病毒癌基因中缺失的5'区。第六个v-myb外显子下游的序列不包括在本研究分析的17 kbp DNA序列中。比较正常c-myb克隆与浆细胞样淋巴肉瘤中重排的对应克隆的结构，发现重排发生在该位点是病毒整合的结果。目前的研究表明，这样的病毒插入中断的c-myb编码区在一个区域中观察到的相同的产生的禽成髓细胞瘤病毒的v-myb基因，并在合成的mRNA，缺乏相同的5'编码区。
Bacteriophage libraries of mouse DNA were screened for sequences homologous to the v-myb oncogene and two overlapping clones containing the v-myb related region were isolated. Restriction enzyme mapping, heteroduplex analysis and nucleotide sequence analysis revealed the presence of nine exons. Six of these exons are homologous to the v-myb region while the other three exons are derived from the 5' region which is deleted in the viral oncogene. The sequences downstream to the sixth v-myb exon are not included in the 17 kbp of DNA sequences analyzed in this study. Comparison of the structure of the normal c-myb clone with its rearranged couterpart present in plasmacytoid lymphosarcomas revealed that the rearrangements occur in this locus as a result of viral integration. Present studies demonstrate that such a viral insertion interrupts the c-myb coding region at a region identical to that observed in the generation of the v-myb gene of avian myeloblastosis virus and results in the synthesis of mRNAs that lack the same 5' coding region.