Elemental C, N, and P cell content of individual bacteria collected at the Bermuda Atlantic Time-Series Study (BATS) site
Elemental C, N, and P cell content of individual bacteria collected at the Bermuda Atlantic Time-Series Study (BATS) site
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在百慕大大西洋时间序列研究（BATS）地点采集的单个细菌细胞的 C、N 和 P 元素含量
DOI：
10.4319/lo.2002.47.5.1525
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发表时间：
2002-09-01
影响因子：
4.5
通讯作者：
Knap, AH
中科院分区：
文献类型：
--
作者：
Gundersen, K;Heldal, M;Knap, AH
一段时间以来，准确评估细菌中碳（C）、氮（N）和磷（P）的真实元素细胞含量一直是微生物研究中的主要困难。这项研究是第一次提出单细胞元素C，N和P测量自然种群的细菌从马尾藻海使用透射电子显微镜（TEM）配备了X射线探测器单元。元素细胞含量显示出作为细胞大小的幂函数的最佳拟合，并且较小的细菌比较大的细菌具有显著更多的元素C、N和P/细胞体积（比例因子a < 1）。相反，沿海，半咸水，和淡水系统，在这项研究中收集的细菌似乎有一个摩尔元素P：N接近Redfield比为0.063（N：P = 16）。本研究中分析的每个细胞体积的碳的几何平均值（148 fg C μm（-3））高于沿海和半咸水系统报告的任何其他估计值。从丰度估计值计算的细菌C总量需要平均转换因子，因此选择代表性的细菌细胞体积至关重要。我们能够证明生物量评估的结果高度依赖于细胞特异性转换因子的选择。通过应用细胞体积以前报道的BATS网站，平均0-250在1991年和1996年之间的细菌C的深度整合范围为1.7和2.5倍，比其他最近的生物量估计这些沃茨。
Accurate assessments of the true elemental cellular content of carbon (C), nitrogen (N), and phosphorus (P) in bacteria have been a major difficulty in microbial research for some time. This study is the first to present single-cell elemental C, N, and P measurements on natural populations of bacteria from the Sargasso Sea using a transmission electron microscope (TEM) equipped with an X-ray detector unit. Elemental cell content showed a best fit as a power function of the cell size, and smaller bacteria had significantly more elemental C, N, and P per cell volume than the larger ones (scaling factor a < 1). Contrary to coastal, brackish, and freshwater systems, the bacteria collected in this study appeared to have a molar elemental P: N close to the Redfield ratio of 0.063 (N: P = 16). The geometric mean of C per cell volume analyzed in this study (148 fg C μm(-3)) was higher than any other estimates reported from coastal and brackish water systems. Total amount of bacterial C calculated from abundance estimates requires an average conversion factor, and the choice of a representative bacterial cell volume is therefore critical. We were able to demonstrate that the outcome of biomass assessments is highly dependant on the choice of cell-specific conversion factors. By applying cell volumes previously reported from the BATS site, the average 0-250 In depth integration of bacterial C between 1991 and 1996 ranged between 1.7 and 2.5 times less than other recent biomass estimates for these waters.