Proteasome activity and biological properties of normal prion protein: a comparison between young and aged cattle.
Proteasome activity and biological properties of normal prion protein: a comparison between young and aged cattle.
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DOI：
10.1292/jvms.10-0149
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发表时间：
2010-08
期刊：
影响因子：
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通讯作者：
Y. Yoshioka;N. Ishiguro;Y. Inoshima
中科院分区：
文献类型：
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作者：
Y. Yoshioka;N. Ishiguro;Y. Inoshima
非典型牛海绵状脑病（非典型BSE）最近在包括日本在内的几个国家被发现。大多数非典型BSE病例报告发生在8岁以上的牛中。为了阐明年龄与非典型BSE发生之间的关系，我们通过酶联免疫吸附试验和免疫印迹法研究了年轻和老年牛脑匀浆中细胞朊蛋白（PrP（C））的生理特性和数量。青年牛和老年牛脑匀浆中PrP（C）的含量无显著差异，但老年牛洗涤剂不溶性部分中PrP（C）的含量显著高于青年牛。青年牛和老年牛的糖基化形式和蛋白酶K敏感性均无显著差异。与青年牛相比，老年牛脑中脂褐素色素沉积和蛋白酶体活性降低33%。
Atypical bovine spongiform encephalopathy (atypical BSE) has recently been identified in several countries including Japan. Most cases of atypical BSE have been reported in cattle older than 8 years of age. To clarify the association between age and occurrence of atypical BSE, we investigated both the physiological properties and amount of cellular prion protein (PrP(C)) in brain homogenates from young and aged cattle by enzyme-linked immunosorbent assay and immunoblotting. The amount of PrP(C) in the brain homogenates was not significantly different between young and aged cattle, but the amount in the detergent-insoluble fraction in the aged cattle was significantly higher than that of young cattle. Significant differences were observed in neither of the glycosylation forms nor in proteinase K sensitivity in young and aged cattle. Age-related changes included deposition of lipofuscin pigment and a decrease of 33% in proteasome activity in the brains of aged cattle compared to that of young cattle.