DNA-Origami-Templated Growth of Multilamellar Lipid Assemblies
DNA-Origami-Templated Growth of Multilamellar Lipid Assemblies
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DOI：
10.1002/anie.202006044
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发表时间：
2020-11-09
影响因子：
16.6
通讯作者：
Kostiainen, Mauri A.
中科院分区：
文献类型：
--
作者：
Julin, Sofia;Nonappa;Kostiainen, Mauri A.
脂质是细胞区室中的重要构件，因此它们自组装成明确定义的分级结构已获得越来越多的关注。阳离子脂质和非结构化DNA可以共组装成高度有序的结构（脂质复合物），但其潜在的应用仍然有限。使用支架DNA折纸纳米结构可以帮助解决这些缺点。在这里，我们有复杂的DNA折纸与阳离子脂质1，2-dioleoly-3-trimethylammonium-propane（DOTAP）和研究它们的自组装驱动的静电和疏水相互作用。结果表明，DNA折纸功能作为模板的多层脂质结构的生长和DNA折纸嵌入在形成的脂质基质。此外，发现脂质包裹可以显着保护DNA折纸免受核酸酶消化。所提出的复合策略适用于广泛的基于DNA的模板，因此可以在构建细胞膜相关组件中找到用途。
Lipids are important building blocks in cellular compartments, and therefore their self-assembly into well-defined hierarchical structures has gained increasing interest. Cationic lipids and unstructured DNA can co-assemble into highly ordered structures (lipoplexes), but potential applications of lipoplexes are still limited. Using scaffolded DNA origami nanostructures could aid in resolving these drawbacks. Here, we have complexed DNA origami together with a cationic lipid 1,2-dioleoly-3-trimethylammonium-propane (DOTAP) and studied their self-assembly driven by electrostatic and hydrophobic interactions. The results suggest that the DNA origami function as templates for the growth of multilamellar lipid structures and that the DNA origami are embedded in the formed lipid matrix. Furthermore, the lipid encapsulation was found to significantly shield the DNA origami against nuclease digestion. The presented complexation strategy is suitable for a wide range of DNA-based templates and could therefore find uses in construction of cell-membrane-associated components.