Electroacupuncture suppresses myostatin gene expression: cell proliferative reaction in mouse skeletal muscle
Electroacupuncture suppresses myostatin gene expression: cell proliferative reaction in mouse skeletal muscle
复制标题
DOI：
10.1152/physiolgenomics.00057.2006
复制
发表时间：
2007-07-18
影响因子：
4.6
通讯作者：
Maeda, Eiichi
中科院分区：
文献类型：
--
作者：
Takaoka, Yutaka;Ohta, Mika;Maeda, Eiichi
补充和替代医学（CAM）可以为患者提供传统医学的替代方案，但需要对其疗效进行评估。 CAM 方法之一，即电针 (EA) 治疗，是一种利用低频微电流刺激针灸针的方法。为了研究短期 EA 的效果，我们评估了长达 24 小时的小鼠骨骼肌中 EA 诱导的基因差异表达。然后我们使用RT-PCR来确认六个差异表达基因的表达模式。对其转录控制区的生物信息学分析表明，EA诱导基因具有许多与细胞分化、细胞增殖、肌肉修复和增生相关的共同结合基序。这些结果表明电针治疗可能会诱导骨骼肌细胞增殖。为了验证这种可能性，我们每天使用 EA 刺激小鼠骨骼肌长达 1 个月，并检查长期效果。免疫组织化学分析表明，经EA处理1mo的肌细胞的细胞核，尤其是卫星细胞的细胞核，与抗人PCNA发生反应。此外，每日 EA 治疗 1 周可抑制编码肌肉生长抑制素（肌肉卫星细胞中的生长抑制因子）的基因表达； EA 处理 1 个月导致该基因更加明显的抑制。这些分子发现有力地证明，电针治疗可抑制肌肉生长抑制素的表达，从而导致卫星细胞相关的增殖反应和骨骼肌修复。
Complementary and alternative medicine ( CAM) may provide patients with an alternative to traditional medicine, but an assessment of its efficacy is required. One CAM method, electroacupuncture ( EA) treatment, is a maneuver that utilizes stimulation of acupuncture needles with a low-frequency microcurrent. To study the effect of short-term EA, we evaluated the differential expression of genes induced by EA in mouse skeletal muscle for up to 24 h. We then used RT-PCR to confirm the expression patterns of six differentially expressed genes. Bioinformatics analysis of their transcription control regions showed that EA-inducible genes have numerous common binding motifs that are related to cell differentiation, cell proliferation, muscle repair, and hyperplasia. These results suggested that EA treatment may induce cell proliferation in skeletal muscle. To verify this possibility, we used EA to stimulate mouse skeletal muscle daily for up to 1 mo and examined the long-term effects. Immunohistochemical analysis showed that nuclei of muscle cells treated with EA for 1 mo, especially nuclei of satellite cells, reacted with anti-human PCNA. Also, expression of the gene encoding myostatin, which is a growth repressor in muscle satellite cells, was suppressed by daily EA treatment for 1 wk; EA treatment for 1 mo resulted in more marked suppression of the gene. These molecular findings constitute strong evidence that EA treatment suppresses myostatin expression, which leads to a satellite cell-related proliferative reaction and repair in skeletal muscle.