Endothelium in vitro: A review of human vascular endothelial cell lines for blood vessel-related research
Endothelium in vitro: A review of human vascular endothelial cell lines for blood vessel-related research
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DOI：
10.1023/a:1012259529167
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发表时间：
2001-01-01
期刊：
影响因子：
9.8
通讯作者：
Mulder, Nanno H.
中科院分区：
文献类型：
--
作者：
Bouis, Diane;Hospers, Geke A. P.;Mulder, Nanno H.
内皮细胞（EC）目前被用作各种生理和病理过程的体外模型系统，特别是在血管生成研究中。原发EC的寿命有限，并且由于其多供体来源而表现出不同批次的特征。近年来，许多集团建立了EC生产线。本文综述了目前可用的血管EC管线的优点和缺点。其目的是帮助研究者决定哪种细胞系最符合他或她的研究目标。真正永生化的细胞系通常比寿命延长的内皮细胞具有更好的特性和更稳定的内皮特性。目前最具特征的大血管和微血管EC系分别是EA.hy926和HMEC-1。
Endothelial cells (EC) are currently used as in vitro model systems for various physiological and pathological processes, especially in angiogenesis research. Primary EC have a limited lifespan and display characteristics that differ from batch to batch due to their multidonor origin. In recent years many groups have established EC lines. This Review gives an overview of the advantages and disadvantages of currently available vascular EC lines. Its aim is to help the investigator to decide which cell line matches his or her research goal best. Truly immortalized cell lines are generally better characterized and more stable in their endothelial traits than EC that were given an extended life span. Presently the best characterized macro- and micro-vascular EC lines are EA.hy926 and HMEC-1, respectively.