Metabolite identification and pharmacokinetic study of platycodi radix (Jiegeng) in vivo
Metabolite identification and pharmacokinetic study of platycodi radix (Jiegeng) in vivo
复制标题
桔梗体内代谢鉴定及药动学研究
DOI：
10.1039/c7ra04814a
复制
发表时间：
2017-01-01
期刊：
影响因子：
3.9
通讯作者：
Bai, Gang
中科院分区：
文献类型：
--
作者：
Tang, Zhongyao;Hou, Yuanyuan;Bai, Gang
由于桔梗皂甙的活性，桔梗通常被用作通经药物，辅助治疗呼吸系统疾病。在这项研究中，使用超高压液相色谱四极杆飞行时间质谱（UPLC-Q/TOF-MS）和基于碎片行为的策略，在总皂苷中总共鉴定出13种桔梗苷，其中在单次口服总皂苷后的大鼠血浆中发现了其中7种。已证明次生桔梗苷是体内吸收的主要皂苷。从总皂苷中分离出主要吸收的桔梗苷，3-O-β-D-吡喃葡萄糖基桔梗酸 (GPA) 和 3-O-β-D-吡喃葡萄糖基桔梗皂甙元 (GP)，并通过液相色谱三重四极杆质谱 (LC-MS/MS) 评估小鼠体内的药代动力学。 The results of the pharmacokinetic analysis indicated that GPA and GP were quickly absorbed with short T-max (
Platycodi radix (Jiegeng) is commonly used as a channel ushering drug for assistance in treating respiratory diseases, because of the activities of saponins that are called platycosides. In this study, using ultra high pressure liquid chromatography with quadrupole time of flight mass spectrometry (UPLC-Q/TOF-MS) and a strategy based on fragmentation behaviors, a total of thirteen platycosides were identified in the total saponins, and seven of these were found in rat plasma following a single oral dose of total saponins. The secondary platycosides were proved to be the main absorbed saponins in vivo. The major absorbed platycosides, 3-O-beta-D-glucopyranosylplatyconic acid (GPA) and 3-O-beta-D-glucopyranosylplatycodigenin (GP), were isolated from total saponins and the pharmacokinetics were evaluated in mice by liquid chromatography with triple quadrupole mass spectrometry (LC-QQQ). The results of the pharmacokinetic analysis indicated that GPA and GP were quickly absorbed with short T-max (