In vivo simultaneous morphological and biochemical optical imaging of oral epithelial cancer.
In vivo simultaneous morphological and biochemical optical imaging of oral epithelial cancer.
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DOI：
10.1109/tbme.2010.2060485
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发表时间：
2010-10
期刊：
影响因子：
--
通讯作者：
Brandon JL
中科院分区：
文献类型：
--
作者：
Jo JA;Applegate BE;Park J;Shrestha S;Pande P;Gimenez-Conti IB;Brandon JL
早期发现癌症是降低发病率和死亡率的关键。形态学和化学生物标志物预示着从正常组织到癌组织的转变。我们已经开发出一种非侵入性成像系统，将光学相干断层扫描（OCT）和荧光寿命成像显微镜（FLIM）到一个单一的光学系统的第一次，以获得两套生物标志物。OCT可以提供具有高分辨率的组织形态学图像，而FLIM可以提供生化组织图。共配准的OCT卷和FLIM图像已同时在口腔癌的体内仓鼠颊囊模型中获得。OCT图像指示癌症的形态学生物标志物，包括上皮层的增厚和分层结构的丧失。FLIM图像显示化学生物标志物，包括烟酰胺腺嘌呤二核苷酸增加和胶原蛋白排放减少。虽然这两组生物标志物都可以区分正常和癌性组织，但我们相信它们的组合将能够区分具有一些指示的生物标志物的良性病变，例如，疤痕或炎症。
Early detection of cancer is key to reducing morbidity and mortality. Morphological and chemical biomarkers presage the transition from normal to cancerous tissue. We have developed a noninvasive imaging system incorporating optical coherence tomography (OCT) and fluorescence lifetime imaging microscopy (FLIM) into a single optical system for the first time, in order to acquire both sets of biomarkers. OCT can provide morphological images of tissue with high resolution, while FLIM can provide biochemical tissue maps. Coregistered OCT volumes and FLIM images have been acquired simultaneously in an in vivo hamster cheek pouch model of oral cancer. The OCT images indicate morphological biomarkers for cancer including thickening of the epithelial layer and loss of the layered structure. The FLIM images indicate chemical biomarkers including increased nicotinamide adenine dinucleotide and reduced collagen emission. While both sets of biomarkers can differentiate normal and cancerous tissue, we believe their combination will enable the discrimination of benign lesions possessing some of the indicated biomarkers, e.g., scarring or inflammation.