STRUCTURE OF THE SINUS-LINING CELLS IN THE POPLITEAL LYMPH-NODE OF THE RABBIT
STRUCTURE OF THE SINUS-LINING CELLS IN THE POPLITEAL LYMPH-NODE OF THE RABBIT
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DOI：
10.1002/ar.1092120412
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发表时间：
1985-01-01
影响因子：
--
通讯作者：
RAVIOLA, E
中科院分区：
文献类型：
--
作者：
COMPTON, CC;RAVIOLA, E
本文应用电镜观察了家兔腘淋巴结窦壁的结构。在边缘窦中，内皮细胞通过缝隙连接、粘附点和表面特化连接，其特征在于相邻膜的局灶性接近而不融合。它们具有大量的简单和复合的质膜未被覆盖的内陷，这些内陷被具有中央增厚的隔膜封闭。跨窦腔的组织束由含有胶原和弹性纤维的纤维核心组成，被与构成窦外壁的内皮细胞相同的内皮细胞包围。皮质窦独立运行的小梁被确定利用的事实，即他们的内皮细胞积累淋巴传递的铁蛋白，其内腔概述了静脉注射辣根过氧化物酶。它们由扁平、连续的内皮细胞排列，缺乏腔束。髓窦壁由内皮细胞和巨噬细胞组成。内皮细胞通过专门的连接点相互连接，并且含有比皮质中更少的质膜小泡;此外，在其细胞质中存在致密颗粒。巨噬细胞粘附于内皮细胞表面;通常，表征内皮细胞之间界面的连接特化均不将内皮细胞连接至巨噬细胞。然而，在沿着与内皮接触区域的点处，巨噬细胞的质膜被蓬松的细胞质物质的附着斑装饰。窦内皮细胞像体内其他地方的血管内皮细胞一样缓慢积累淋巴传递的铁蛋白，而巨噬细胞既含有铁蛋白又含有吞噬的红细胞。
The structure of the sinus walls in the popliteal lymph node of the rabbit was studied with the EM. In the marginal sinus, the endothelial cells are connected by gap junctions, puncta adherentia, and surface specializations characterized by focal approximation of the adjoining membranes without fusion. They possess large numbers of simple and compound uncoated invaginations of the plasma membrane that are closed by a diaphragm with a central thickening. The tissue strands that straddle the lumen of the sinus consist of a fibrous core containing both collagen and elastic fibers, surrounded by endothelial cells identical to those composing the outer sinus wall. Cortical sinuses that run independently of the trabeculae were identified by exploiting the fact that their endothelial cells accumulate lymph-borne ferritin, and their lumen is outlined by horseradish peroxidase administered intravenously. They are lined by a flattened, continuous endothelium and lack luminal strands. The walls of the medullary sinuses consist of endothelial cells and macrophages. The endothelial cells are interconnected by specialized junctions and contain fewer plasmalemmal vesicles than in the cortex; furthermore, dense granules are present in their cytoplasm. Macrophages adhere to the surface of the endothelial cells; typically, none of the junctional specializations that characterize the interface between endothelial cells connect endothelial cells to macrophages. However, at points along the contact region with the endothelium, the plasmalemma of the macrophage is decorated by an attachment plaque of fluffy cytoplasmic material. Sinus endothelial cells slowly accumulate lymph-borne ferritin like vascular endothelial cells elsewhere in the body, whereas macrophages contain both ferritin and engulfed erythrocytes.