Reduced Protein Adsorption by Osmolytes
Reduced Protein Adsorption by Osmolytes
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DOI：
10.1021/la2010908
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发表时间：
2011-06-07
期刊：
影响因子：
3.9
通讯作者：
Czeslik, Claus
中科院分区：
文献类型：
--
作者：
Evers, Florian;Steitz, Roland;Czeslik, Claus
渗透剂是影响渗透作用的物质，细胞利用渗透剂来适应环境压力。在这里，我们报告了一些渗透剂对固液界面蛋白质吸附影响的中子反射率研究。牛核糖核酸酶 A (RNase) 和牛胰岛素被用作在亲水性二氧化硅和疏水性聚苯乙烯表面吸附的模型蛋白。根据中子反射率数据，在不存在和存在尿素、海藻糖、蔗糖和甘油的情况下，根据层厚度、蛋白质堆积密度和吸附蛋白质质量来表征吸附蛋白质层。所有数据都表明这些非离子助溶剂对蛋白质吸附程度有明显的影响。例如，1 M 蔗糖导致二氧化硅表面上的 RNase 吸附量减少 39%，聚苯乙烯表面上的 RNase 吸附量减少 71%。发现海藻糖表现出与蔗糖相似的活性。吸附蛋白质质量的变化可归因于吸附层中蛋白质堆积密度的降低。此外，我们研究了在甘油不存在和存在的情况下疏水表面上的胰岛素吸附。在4M甘油存在下，胰岛素吸附程度甚至降低80%。这项研究的结果表明，非离子助溶剂可用于调节和控制水性固体界面上的非特异性蛋白质吸附，这可能与生物医学应用相关。
Osmolytes are substances that affect osmosis and are used by cells to adapt to environmental stress. Here, we report a neutron reflectivity study on the influence of some osmolytes on protein adsorption at solid liquid interfaces. Bovine ribonuclease A (RNase) and bovine insulin were used as model proteins adsorbing at a hydrophilic silica and at a hydrophobic polystyrene surface. From the neutron reflectivity data, the adsorbed protein layers were characterized in terms of layer thickness, protein packing density, and adsorbed protein mass in the absence and presence of urea, trehalose, sucrose, and glycerol. All data point to the clear effect of these nonionic cosolvents on the degree of protein adsorption. For example, 1 M sucrose leads to a reduction of the adsorbed amount of RNase by 39% on a silica surface and by 71% on a polystyrene surface. Trehalose was found to exhibit activity similar to that of sucrose. The changes in adsorbed protein mass can be attributed to a decreased packing density of the proteins in the adsorbed layers. Moreover, we investigated insulin adsorption at a hydrophobic surface in the absence and presence of glycerol. The degree of insulin adsorption is decreased by even 80% in the presence of 4 M of glycerol. The results of this study demonstrate that nonionic cosolvents can be used to tune and control nonspecific protein adsorption at aqueous solid interfaces, which might be relevant for biomedical applications.