THE METABOLIC PATHWAY GENERATING P3, AN A-BETA-PEPTIDE FRAGMENT, IS PROBABLY NON-AMYLOIDOGENIC
THE METABOLIC PATHWAY GENERATING P3, AN A-BETA-PEPTIDE FRAGMENT, IS PROBABLY NON-AMYLOIDOGENIC
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DOI：
10.1006/bbrc.1994.2527
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发表时间：
1994-10-28
影响因子：
3.1
通讯作者：
NORDSTEDT, C
中科院分区：
文献类型：
--
作者：
NASLUND, J;JENSEN, M;NORDSTEDT, C
阿尔茨海默A β淀粉样蛋白前体蛋白通过至少两种分泌途径代谢。一个产生A -肽，另一个产生n端截断的A -片段，称为p3，被认为是非淀粉样变性的。然而，缺乏直接证据。我们已经着手合成和纯化p3。纯p3在体外聚合，形成晶格，其超微结构与a β的线性原纤维不同。与淀粉样蛋白不同，聚合后的p3不结合硫黄酮t。因此，A β分子n端部分的氨基酸是形成典型淀粉样蛋白原纤维所必需的，产生p3的代谢途径可能是非淀粉样蛋白形成的。(C) 1994学术出版社，Inc.
The Alzheimer A beta amyloid precursor protein is metabolized by at least two secretory pathways. One generates the A beta peptide and the other a N-terminally truncated A beta fragment termed p3 that is considered non-amyloidogenic. However, direct evidence is missing. We have undertaken to synthesize and purify p3. Pure p3 polymerizes in vitro, forming a lattice with an ultrastructure distinct from the linear fibrils of A beta. In contrast to amyloid, polymerized p3 does not bind thioflavine T. It is therefore concluded that amino acids in the N-terminal part of the A beta molecule are required for formation of typical amyloid fibrils and that the metabolic pathway generating p3 probably is non-amyloidogenic. (C) 1994 Academic Press, Inc.