Phospholipid class as a determinant in docosahexaenoic acid's effect on tumor cell viability.
Phospholipid class as a determinant in docosahexaenoic acid's effect on tumor cell viability.
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发表时间：
1996-09
影响因子：
2
通讯作者：
M. Zerouga;W. Stillwell;J. Stone;A. Powner;L. Jenski
中科院分区：
文献类型：
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作者：
M. Zerouga;W. Stillwell;J. Stone;A. Powner;L. Jenski
在这里，我们探讨了omega-3脂肪酸二十二碳六烯酸（DHA）在小鼠白血病细胞（T27A）中的掺入如何改变膜结构和功能。当细胞在补充DHA的培养基中培养时，DHA与磷脂酰乙醇胺（PE）结合迅速且优先，与磷脂酰胆碱（PC）结合较少且较慢。低浓度的DHA比中性脂更倾向于PE，但在中性脂中DHA的过量积累超过磷脂。在明显没有脂质过氧化作用的情况下，高DHA水平降低了细胞生长。为了研究DHA磷脂类的重要性，将细胞与18:0,22:6 PE或18:0,22:6 PC的脂质囊泡融合。含dha的PC囊泡对细胞活力产生剂量依赖性的降低，而含pe的囊泡对细胞活力的影响很小，尽管它们看起来更具有融合性。这些结果引发了一个有趣的猜测，即T27A细胞可以安全地在PE中积累DHA，但如果过量的DHA被纳入PC，则易受伤害。
Here we explore how incorporation of the omega-3 fatty acid docosahexaenoic acid (DHA) into murine leukemia cells (T27A) may alter membrane structure and function. When cells were cultured in DHA-supplemented medium, DHA incorporated rapidly and preferentially into phosphatidyl-ethanolamine (PE), with lesser and slower incorporation into phosphatidylcholine (PC). DHA at low concentrations preferred PE over neutral lipids, but in DHA excess accumulation in neutral lipids outstripped that of phospholipids. High DHA levels reduced cell growth in the apparent absence of lipid peroxidation. To study the importance of DHA's phospholipid class, cells were fused with lipid vesicles of either 18:0, 22:6 PE or 18:0, 22:6 PC. DHA-containing PC vesicles produced a dose-dependent decrease in cell viability, whereas PE-containing vesicles had little effect although they appeared more fusogenic. These results provoke the interesting speculation that T27A cells can safely accumulate DHA in PE, but are vulnerable if excessive DHA is incorporated into PC.