Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression
Using the Flp-In™ T-Rex™ System to Regulate GPCR Expression
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DOI：
10.1007/978-1-61779-126-0_2
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发表时间：
2011-01-01
期刊：
影响因子：
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通讯作者：
Milligan, Graeme
中科院分区：
文献类型：
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作者：
Ward, Richard J.;Alvarez-Curto, Elisa;Milligan, Graeme
允许将感兴趣的基因（GOI）（例如G蛋白偶联受体（GPCR））整合到基因组上的特定位点中的基于细胞的系统的开发已经使得能够容易且有效地表达此类蛋白质的哺乳动物细胞系的产生。Flp-In（TM）稳定细胞系是等基因的，因此蛋白质表达在整个细胞群中是恒定的。对Flp-In（TM）系统（Flp-In（TM）T-Rex（TM））的有用添加允许通过向细胞培养基中添加小分子诱导剂来控制该表达。如本文所述产生的稳定细胞系可用于受体药理学信号传导和寡聚化的研究中，具有很大的优势。
The development of a cell-based system that allows the integration of a gene of interest (GOI), such as a G protein-coupled receptor (GPCR), into a specific site on the genome, has made the generation of mammalian cell lines able to express such proteins easy and efficient. Flp-In (TM) stable cell lines are isogenic and hence protein expression is constant across a population of cells. A useful addition to the Flp-In (TM) system (Flp-In (TM) T-Rex (TM)) allows this expression to be controlled by the addition of a small molecule inducer to the cell culture medium. Stable cell lines generated as described here can be used to great advantage in the study of receptor pharmacology signalling and oligomerisation.