Neonatal Vitamin A Supplementation and Vitamin A Status Are Associated with Gut Microbiome Composition in Bangladeshi Infants in Early Infancy and at 2 Years of Age
Neonatal Vitamin A Supplementation and Vitamin A Status Are Associated with Gut Microbiome Composition in Bangladeshi Infants in Early Infancy and at 2 Years of Age
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DOI：
10.1093/jn/nxz034
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发表时间：
2019-06-01
影响因子：
4.2
通讯作者：
Stephensen, Charles B.
中科院分区：
文献类型：
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作者：
Huda, M. Nazmul;Ahmad, Shaikh M.;Stephensen, Charles B.
背景：婴儿期是建立肠道微生物组的关键时期。这一过程可能受到维生素A(VA)状态的影响，因为VA影响肠道免疫和上皮完整性，这些因素反过来可以调节微生物组的发育。目的：本研究的目的是确定新生儿补充VA(VAS)是否影响婴儿早期(6-15周)或晚期(2岁)双歧杆菌(有益的共生体)或变形杆菌(一种含有肠道病原体的门)的丰度。次要目标是确定维生素A是否影响其他细菌类群的丰度，并确定通过测量血浆视黄醇来评估VA状态是否与细菌丰度相关。方法：306名孟加拉婴儿按性别和出生体重状况(高于或低于中位数)随机接受出生48小时内接受1次VA剂量(50，0IU)或安慰剂的治疗。通过16S rRNA基因测序分析了属及属以上的相对丰度。结果：线性回归分析显示，男婴早期双歧杆菌丰度(中位数，1/3四分位数；0.67，0.52/0.78)低于女童(0.73，0.60/0.80；P=0.003)，但男童(0.69，0.55/0.78)高于安慰剂组(0.65，0.44/0.77；P=0.039)。但在女孩中未见这种差异(VAS 0.71，0.54/0.80；安慰剂0.75，0.63/0.81；P=0.25)。VAS不影响变形杆菌的丰度。对婴儿后期的女孩来说，VA状态也与性别相关，包括血浆视黄醇与放线菌(含双歧杆菌门)和阿克曼属(另一种可能有益于健康的共生生物)呈正相关。结论：婴儿时期较好的VA状态可能通过促进健康微生物区系的建立而影响婴儿和以后生活的健康。VA的这种假定效应可能在男孩和女孩之间有所不同。这项试验在Clinicaltrials.gov上注册为NCT02027610。
Background: Infancy is a crucial period for establishing the intestinal microbiome. This process may be influenced by vitamin A (VA) status because VA affects intestinal immunity and epithelial integrity, factors that can, in turn, modulate microbiome development.Objectives: The aim of this study was to determine if neonatal VA supplementation (VAS) affected the abundance of Bifidobacterium, a beneficial commensal, or of Proteobacteria, a phylum containing enteric pathogens, in early (6-15 wk) or late (2 y) infancy. Secondary objectives were to determine if VAS affected the abundance of other bacterial taxa, and to determine if VA status assessed by measuring plasma retinol was associated with bacterial abundance.Methods: Three hundred and six Bangladeshi infants were randomized by sex and birthweight status (above/below median) to receive 1 VA dose (50,000 IU) or placebo within 48 h of birth. Relative abundance at the genus level and above was assessed by 16S rRNA gene sequencing. A terminal restriction fragment-length polymorphism assay was used to identify Bifidobacterium species and subspecies at 6 wk.Results: Linear regression showed that Bifidobacterium abundance in early infancy was lower in boys (median, 1st/3rd quartiles; 0.67, 0.52/0.78) than girls (0.73, 0.60/0.80; P = 0.003) but that boys receiving VAS (0.69, 0.55/0.78) had higher abundance than boys receiving placebo (0.65, 0.44/0.77; P = 0.039). However this difference was not seen in girls (VAS 0.71, 0.54/0.80; placebo 0.75, 0.63/0.81; P = 0.25). VAS did not affect Proteobacteria abundance. Sex-specific associations were also seen for VA status, including positive associations of plasma retinol with Actinobacteria (the phylum containing Bifidobacterium) and Akkermansia, another commensal with possible health benefits, for girls in late infancy.Conclusions: Better VA status in infancy may influence health both in infancy and later in life by promoting the establishment of a healthy microbiota. This postulated effect of VA may differ between boys and girls. This trial was registered at clinicaltrials.gov as NCT02027610.