Mechanisms of anti-influenza activity of surfactant proteins A and D: Comparison with serum collectins
Mechanisms of anti-influenza activity of surfactant proteins A and D: Comparison with serum collectins
复制标题
DOI：
10.1152/ajplung.1997.273.6.l1156
复制
发表时间：
1997-12-01
影响因子：
4.9
通讯作者：
Crouch, EC
中科院分区：
文献类型：
--
作者：
Hartshorn, KL;White, MR;Crouch, EC
本研究首次直接比较了凝集素表面活性蛋白（SP）A和SP-D、甘露糖结合凝集素（MEL）和连接素的抗甲型流感病毒（IAV）活性。SP-D，MEL，和conglutinin抑制IAV血凝活性的效力比SP-A和不同的机制。虽然分离的三聚体SP-D碳水化合物识别域抑制血凝活性，制备的SP-D也含有胶原结构域和NH 2末端引起更大的抑制。与SP-A（或非多聚化SP-D）相反，缺乏N-连接的附件不影响多聚化SP-D与IAV的相互作用。SP-D、SP-A和伴发蛋白通过形成大量病毒聚集体引起病毒沉淀，而MBL形成不沉淀的较小尺寸的聚集体。所有的胶原凝集素增强IAV结合中性粒细胞，然而，在MBL的情况下，这种效果是温和的结合增强SP-D或conglutinin诱导相比。这些研究阐明了SP-D抑制病毒的结构要求，并揭示了胶原凝集素抗IAV活性机制的显着差异。
The present study provides the first direct comparison of anti-influenza A virus (IAV) activities of the collectins surfactant protein (SP) A and SP-D, mannose-binding lectin (MEL), and conglutinin. SP-D, MEL, and conglutinin inhibited IAV hemagglutination activity with a greater potency than and by a distinct mechanism from SP-A. Although isolated trimeric SP-D carbohydrate recognition domains inhibited hemagglutination activity, preparations of SP-D also containing the collagen domain and NH2 terminus caused greater inhibition. In contrast to SP-A (or nonmultimerized SP-D), absence of the N-linked attachment did not effect interactions of multimerized SP-D with IAV. SP-D, SP-A, and conglutinin caused viral precipitation through formation of massive viral aggregates, whereas MBL formed aggregates of smaller size that did not precipitate. All of the collectins enhanced IAV binding to neutrophils; however, in the case of MBL, this effect was modest compared with the binding enhancement induced by SP-D or conglutinin. These studies clarify the structural requirements for viral inhibition by SP-D and reveal significant differences in the mechanisms of anti-IAV activity among the collectins.