Modular Platform of Carbohydrates-modified Supramolecular Polymers Based on Dendritic Peptide Scaffolds.
Modular Platform of Carbohydrates-modified Supramolecular Polymers Based on Dendritic Peptide Scaffolds.
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基于树枝状肽支架的碳水化合物修饰超分子聚合物模块化平台
DOI：
10.1021/acspolymersau.2c00032
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发表时间：
2022-12-14
期刊：
影响因子：
--
通讯作者：
Chen, Guosong
中科院分区：
文献类型：
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作者：
Li, Long;Wu, Libin;Urschbach, Moritz;StraSSburger, David;Liu, Xiaomei;Besenius, Pol;Chen, Guosong
由于碳水化合物在介导细胞间通讯和调节免疫反应方面具有重要功能，因此显示多价碳水化合物的糖肽超分子聚合物特别适合用于免疫相关的生物材料。然而，碳水化合物的多样性和复杂性限制了糖肽超分子单体的产生。因此，呈现各种碳水化合物，特别是更复杂的低聚糖的模块化平台是非常理想的，但仍未得到充分开发。本文首先制备了自组装成球形纳米粒子和蠕虫状纳米粒子的线性两亲性糖肽。此外，通过重新设计分子骨架，树突糖肽可以自组装成均匀的纳米棒，从而产生具有可变功能的模块化超分子聚合物。体外研究进一步表明，利用各种功能性低聚糖修饰的超分子聚合物，这些聚合物对巨噬细胞没有细胞毒性，并能显著调节促炎细胞因子的产生。这些发现为开发在免疫调节和免疫治疗方面具有潜在应用前景的超分子糖肽生物材料提供了良好的平台。
Glycopeptide supramolecular polymers displaying multivalent carbohydrates are particularly suitable for immune-relevant biomaterials, due to the important functions of carbohydrates in mediating cell-cell communication and modulating immune responses. However, the diversity and complexity of carbohydrates limited the generation of glycopeptide supramolecular monomers. Thereby, a modular platform of presenting various carbohydrates, especially more complex oligosaccharides, is highly desirable but remains underexplored. Here, we first prepared the linear amphiphilic glycopeptides that self-assembled into spherical nanoparticles and worm-like nanoparticles. Furthermore, the dendritic glycopeptides that self-assembled into uniform nanorods were designed to generate modular supramolecular polymers with variable functionality, via redesigning the molecular backbone. With various functional oligosaccharide-modified supramolecular polymers, the in vitro studies further indicated that these polymers were not cytotoxic to macrophages, and significantly modulated the production of proinflammatory cytokines. These findings provide a promising platform to develop supramolecular glycopeptide biomaterials with potential applications in immunomodulation and immunotherapy.