The Escherichia coli retrons Ec67 and Ec86 replace DNA between the cos site and transcription terminator of a 186-related prophage
The Escherichia coli retrons Ec67 and Ec86 replace DNA between the cos site and transcription terminator of a 186-related prophage
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DOI：
10.1006/viro.1996.0228
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发表时间：
1996-05-01
期刊：
影响因子：
3.7
通讯作者：
Egan, B
中科院分区：
文献类型：
--
作者：
Dodd, IB;Egan, B
反转录子是在细菌中发现的不寻常的逆转录酶编码元件。虽然有许多迹象表明，retron是移动的元素，他们的换位尚未观察到。大肠杆菌反转子Ec 67和Ec 86是插入在同一位点的不同反转子，我们进一步表征了该位点，以寻找反转子转座机制的线索。我们确认，通过扩展以前的序列分析，Ec 67和Ec 86插入到原噬菌体相关的大肠杆菌噬菌体186。与最近发表的186 96-2%区域的序列比较表明，反转子已经取代了噬菌体粘性末端位点（cos）和噬菌体DNA包装基因的转录终止子之间的DNA类似的180 bp。这些特征--在插入位点的DNA替换和在转录终止子或DNA切割位点附近的retron连接位置--与其他retron共享，并暗示了retron转座可能发生的方式。(C)出版社：Academic Press，Inc.
Retrons are unusual, reverse transcriptase-encoding elements found in bacteria. Although there are a number of indications that retrons are mobile elements, their transposition has not been observed. The Escherichia coli retrons Ec67 and Ec86 are different retrons inserted at the same site and we have further characterized this site in search of clues to the mechanism of retron transposition. We confirm, by extending previous sequence analysis, that Ec67 and Ec86 are inserted into prophages related to coliphage 186. Comparison with the recently published sequence of the 186 96-2% region indicates that the retrons have replaced similar to 180 bp of DNA between the phage cohesive end site (cos) and the transcription terminator of a phage DNA-packaging gene. These features - DNA replacement at the insertion site and the location of retron junctions near transcription terminators or DNA cleavage sites - are shared with other retrons and suggest ways in which retron transposition might have occurred. (C) 1996 Academic Press, Inc.