STAGE-SPECIFIC EMBRYONIC ANTIGEN INVOLVES ALPHA-1-]3 FUCOSYLATED TYPE-2 BLOOD-GROUP CHAINS
STAGE-SPECIFIC EMBRYONIC ANTIGEN INVOLVES ALPHA-1-]3 FUCOSYLATED TYPE-2 BLOOD-GROUP CHAINS
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DOI：
10.1038/292156a0
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发表时间：
1981-01-01
期刊：
影响因子：
64.8
通讯作者：
EVANS, MJ
中科院分区：
文献类型：
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作者：
GOOI, HC;FEIZI, T;EVANS, MJ
在胚胎发生和分化过程中，发育调节分子可能在细胞相互作用和分选中发挥作用，引起了人们的极大兴趣。已经提出了许多检测抗原的抗血清，这些抗原在早期胚胎细胞中表达，并在分化过程中受到限制，仅在少数成体细胞中表达(见文献1-3)。这些抗血清识别的确切抗原决定簇还没有定义。然而，使用抗小鼠脾细胞的杂交瘤抗体4和感冒凝集素病患者的单抗5的研究表明，两个明确的碳水化合物抗原系统，Forssman和Li抗原，在小鼠早期胚胎中具有阶段特异性表达。我们现在描述阶段特异性胚胎抗原SSEA-1(参考文献1)的证据。6)涉及糖序列Gal1β1→4GlcNAc↑1，3Fucα该决定簇是由血型I或I型抗原的α1→3岩藻糖基化而成，分别是支链或线状低聚糖7-9，由Gal1β1→4GlcNAc单位构成，被称为主要血型抗原的2型前体链10。因此，我们引入了简单糖基化改变的概念作为胚胎抗原阶段特异性表达的基础。
There is much interest in developmentally regulated molecules which may have function in cell interactions and sorting during embryogenesis and differentiation. Numerous antisera have been raised which detect antigens that are expressed in early embryonic cells and become restricted during differentiation, being expressed in only a minority of adult cells (reviewed in refs 1–3). The precise antigenic determinants recognized by such antisera have not been defined. However, studies using a hybri-doma antibody against mouse spleen cells4and monoclonal autoantibodies of patients with cold agglutinin disease5have shown that two defined carbohydrate antigen systems, the Forssman and the li antigens, have stage-specific expression in early mouse embryos. We now describe evidence that the stage-specific embryonic antigen SSEA-1 (ref. 6) involves the carbohydrate sequence Galβ1 → 4GlcNAc ↑1,3FucαThis determinant is formed byα1 → 3 fucosylation of blood group I or i antigens which are branched or linear oligosaccharides, respectively7–9, built of Galβ1 →4GlcNAc units and known as type 2 precursor chains10of the major blood group antigens. Thus, we introduce the concept of simple glycosylation changes as a basis for stage-specific expression of embryonic antigens.