Evaluation of suitable antigens and adjuvant concentration for sublingual immunization to prevent periodontal disease
Evaluation of suitable antigens and adjuvant concentration for sublingual immunization to prevent periodontal disease
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DOI：
10.1002/osi2.1018
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发表时间：
2019-08-01
影响因子：
0.5
通讯作者：
Kurita-Ochiai, Tomoko
中科院分区：
文献类型：
--
作者：
Chang, Emily;Kobayashi, Ryoki;Kurita-Ochiai, Tomoko
由于唾液中存在免疫球蛋白（IG）A抗体（Ab）和龈沟液中存在IgG Ab，口腔由粘膜和全身免疫系统两者主导。因此，允许发展全身和局部免疫应答的粘膜施用的疫苗对于预防口腔感染是最佳的。我们评估了靶向牙龈卟啉单胞菌（GroEL）重组HSP 6 O的粘膜疫苗诱导保护性粘膜免疫应答的能力。作为基础实验，在小鼠中检查了作为抗原（Ag）的GroEL和作为粘膜佐剂的含有未甲基化CpG二核苷酸（CpG ODN）的合成寡核苷酸的最佳浓度。在用GroEL加霍乱毒素或GroEL加CpG ODN的组合的舌下免疫中观察到GroEL特异性免疫应答。GroEL和CpG ODN作为抗原和佐剂舌下免疫的最佳浓度分别为3 μ g/只和25 μ g/只。这些结果表明，舌下给予GroEL加CpG ODN可有效诱导粘膜和全身Ag特异性Ab应答。
The oral cavity is dominated by both mucosal and systemic immune systems, due to the presence of immunoglobulin (Ig)A antibodies (Abs) in saliva and IgG Abs in gingival crevicular fluid. Transmucosally administered vaccines allowing the development of both systemic and local immune responses are thus optimal for preventing oral infections. We assessed the capacity of a mucosal vaccine targeting recombinant HSP6O from Porphyromonas gingivalis (GroEL) to induce protective mucosal immune responses. As the basic experiment, optimal concentrations of GroEL as antigen (Ag) and synthetic oligonucleotides containing unmethylated CpG dinucleotide (CpG ODN) as a mucosal adjuvant for sublingual immunization were examined in mice. GroEL-specific immune responses were observed in sublingual immunization with a combination of GroEL plus cholera toxin or GroEL plus CpG ODN. The optimal concentrations of GroEL and CpG ODN as antigen and adjuvant in sublingual immunization were 3 and 25 mu g per mouse, respectively. These results suggest that the sublingual administration of GroEL plus CpG ODN is effective for inducing mucosal and systemic Ag-specific Ab responses.