The evolutionary patterns of hepatitis C virus subtype 2a and 6a isolates linked to an outbreak in China in 2012.
The evolutionary patterns of hepatitis C virus subtype 2a and 6a isolates linked to an outbreak in China in 2012.
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DOI：
10.1016/j.virol.2015.08.012
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发表时间：
2015-11
期刊：
影响因子：
3.7
通讯作者：
Chen G
中科院分区：
文献类型：
--
作者：
Lu L;An Y;Zou J;Gu L;Zhao Z;Zhang X;Li C;Kurihara C;Hokari R;Itakura J;Kurosaki M;Izumi N;Fu Y;Nakano T;Kato T;Negro F;Chen G
2012年，中国爆发了一场HCV疫情，影响了数百名患者。我们分别对60例和102例患者的HCV亚型2a和6a序列进行了表征，并与82例当地对照和103例校准参考进行了共分析。患者序列的紧密分组与当地对照的多样性形成鲜明对比。根据校准参考品的比例，估计患者分离株的出现时间为采样前2-5年。相比之下，对照品与旧得多的校准参考品混合。对于这两种亚型，可以定义主要和次要集群，其接近程度表明连锁传播。来自研究患者的HCV序列分为三个亚型2a和两个亚型6a簇，以及三个6a孤立分支，代表了八个早期菌株的后代，这些菌株是不同的，否则是散发的。由于重复使用针头的医源性传播，5个菌株被高度选择并优先传播。
An HCV outbreak occurred in 2012 in China, affecting hundreds of patients. We characterized HCV subtype 2a and 6a sequences from 60 and 102 patients, respectively, and co-analyzed with 82 local controls and 103 calibrating references. The close grouping of the patients’ sequences contrasted sharply with the diversity of local controls. Scaled by the calibrating references, the emergence of patients’ isolates was estimated at 2–5 years before sampling. In contrast, the controls intermingled with the calibrating references that were much older. For both subtypes, the major and minor clusters could be defined, with the closeness to indicate linked transmission. HCV sequences from the study patients grouped into three subtype 2a and two subtype 6a clusters, in addition to three 6a solitary branches, representing descendants of eight earlier strains that were distinct and otherwise sporadic. Due to iatrogenic transmission through reusing needles, five strains were highly selected and preferentially spread.