EFFECTS OF HALOTHANE ON TRANSMEMBRANE POTENTIALS, CA-2+ TRANSIENTS, AND PAPILLARY-MUSCLE TENSION IN THE CAT
EFFECTS OF HALOTHANE ON TRANSMEMBRANE POTENTIALS, CA-2+ TRANSIENTS, AND PAPILLARY-MUSCLE TENSION IN THE CAT
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DOI：
10.1152/ajpheart.1986.251.2.h374
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发表时间：
1986-08-01
影响因子：
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通讯作者：
KAMPINE, JP
中科院分区：
文献类型：
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作者：
BOSNJAK, ZJ;KAMPINE, JP
在体外研究了不同起搏频率（60、75、100 和 150 次/分钟）下氟烷对猫乳头肌的影响。在引入1和2个最小肺泡浓度氟烷之前和之后同时测量跨膜电位、细胞内Ca2+瞬变和等长张力。使用气相色谱法测量灌注浴中的麻醉剂浓度。 Ca2+ 敏感光蛋白、水母发光蛋白被显微注射到乳头肌细胞中。水母发光蛋白发光是细胞内游离 [Ca2+] ([Ca2+]i) 的函数。氟烷最显着的作用是在所有起搏速率下，收缩力呈剂量依赖性降低，并且 [Ca2+]i 降低。氟烷的负性肌力作用仅与乳头肌动作电位持续时间的微小变化相关。增加细胞外 [Ca2+] 会导致 [Ca2+]i 增加，并抵消氟烷的负性肌力作用。与对照相比，添加 Ca2+ 通道阻滞剂维拉帕米和氟烷使 [Ca2+]i 显着减少。
The effects of halothane on cat papillary muscle were studied in vitro at different pacing rates (60, 75, 100, and 150 beats/min). Simultaneous measurements of transmembrane potential, intracellular Ca2+ transients, and isometric tension were made before and after introduction of 1 and 2 minimal alveolar concentration halothane. Anesthetic concentrations in the superfusion bath were measured using a gas chromatography procedure. The Ca2+-sensitive photoprotein, aequorin, was microinjected into papillary muscle cells. Aequorin light emission is a function of free intracellular [Ca2+] ([Ca2+]i). The most prominent effects of halothane were a dose-dependent decrease in the contractile force and a decrease in [Ca2+]i, at all pacing rates. The negative inotropic effect of halothane was associated with only small changes in the papillary muscle action potential duration. Increasing the extracellular [Ca2+] produced an increase in [Ca2+]i and counteracted the negative inotropic effects of halothane. Addition of the Ca2+ channel blocker, verapamil, and halothane, produced an additive decrease in [Ca2+]i compared with the control.