Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces
Infectious phage particles packaging antibiotic resistance genes found in meat products and chicken feces
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DOI：
10.1038/s41598-019-49898-0
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发表时间：
2019-09-16
影响因子：
4.6
通讯作者：
Muniesa, Maite
中科院分区：
文献类型：
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作者：
Gomez-Gomez, Clara;Blanco-Picazo, Pedro;Muniesa, Maite
噬菌体可以包装宿主的部分遗传物质，包括抗生素耐药性基因(ARGs)，从而在细菌中迅速传播耐药性。在当地零售商购买的肉类、猪肉、牛肉和鸡肉，以及火腿和摩塔迪拉中，对含有ARGs的噬菌体颗粒进行了评估。用定量聚合酶链式反应(QPCR)分析了10种ARG(BLA(TEM)、BLA(CTX-M-1)、BLA(CTX-M-9)、BLA(OXA-48)、BLA(VIM)、qnrA、qnrS、mecA、Arma和sul1)。在大肠杆菌繁殖实验之前和之后，对这些基因进行了量化，以评估携带ARG的噬菌体颗粒在细菌宿主中感染和繁殖的能力。根据微生物学参数，所有样品均可供食用。在颗粒繁殖后的大多数样品中检测到ARG，表明至少有部分分离的噬菌体颗粒具有感染性，是所有基质中含量最高的ARG，其次是β-内酰胺酶基因。在37份归档鸡盲肠样本的噬菌体DNA组分中也发现了ARG，证实了鸡粪微生物群是ARG的重要储存库，也是肉类中发现的颗粒的可能来源。噬菌体是肉类中基因传播的载体，作为全球抗生素耐药性危机的风险因素，不应被低估。
Bacteriophages can package part of their host's genetic material, including antibiotic resistance genes (ARGs), contributing to a rapid dissemination of resistances among bacteria. Phage particles containing ARGs were evaluated in meat, pork, beef and chicken minced meat, and ham and mortadella, purchased in local retailer. Ten ARGs (bla(TEM), bla(CTX-M-1, )bla(CTX-M-9), bla(OXA-48), bla(VIM), qnrA, qnrS, mecA, armA and sul1) were analyzed by qPCR in the phage DNA fraction. The genes were quantified, before and after propagation experiments in Escherichia coli, to evaluate the ability of ARG-carrying phage particles to infect and propagate in a bacterial host. According to microbiological parameters, all samples were acceptable for consumption. ARGs were detected in most of the samples after particle propagation indicating that at least part of the isolated phage particles were infectious, being sul1 the most abundant ARG in all the matrices followed by beta-lactamase genes. ARGs were also found in the phage DNA fraction of thirty-seven archive chicken cecal samples, confirming chicken fecal microbiota as an important ARG reservoir and the plausible origin of the particles found in meat. Phages are vehicles for gene transmission in meat that should not be underestimated as a risk factor in the global crisis of antibiotic resistance.