Purification and Characterization of Taq Polymerase: A 9-week Biochemistry Laboratory Project for Undergraduate Students
Purification and Characterization of Taq Polymerase: A 9-week Biochemistry Laboratory Project for Undergraduate Students
复制标题
DOI：
10.1002/bmb.20352
复制
发表时间：
2010-01-01
影响因子：
1.4
通讯作者：
Farrow, Melissa A.
中科院分区：
文献类型：
--
作者：
Bellin, Robert M.;Bruno, Mary K.;Farrow, Melissa A.
我们开发了为期 9 周的本科生实验室系列，重点关注水生栖热菌 DNA 聚合酶 (Taq) 的纯化和表征。我们的目标是为生物化学本科生提供一个模拟类似研究体验的整个学期的持续项目，同时每周的程序都集中在一个学习目标上。实验室系列课程已教授了 7 年，并在 2006 年和 2007 年秋季学期完成了对实验室系列课程有效性的调查评估。调查结果的统计分析表明，该实验室系列在教授学生蛋白质纯化和分析的理论和实践方面非常有效，同时在更广泛的科学技能和知识领域也展示了积极的成果。在这些发现中，报告的最大的知识增长与学生对专利法与实验室科学的关系的理解有关，这是一个对现代研究人员非常重要的话题，很容易与 Taq 聚合酶相关的讨论。总体而言，该实验室系列被证明是本科生物和化学专业课程中非常有效的组成部分，并且可能是适合本科生的实验室体验。
We have developed a 9-week undergraduate laboratory series focused on the purification and characterization of Thermus aquaticus DNA polymerase (Taq). Our aim was to provide undergraduate biochemistry students with a full-semester continuing project simulating a research-like experience, while having each week's procedure focus on a single learning goal. The laboratory series has been taught for the past 7 years, and survey-based assessment of the effectiveness of the laboratory series was completed during the 2006 and 2007 fall semesters. Statistical analysis of the survey results demonstrate that the laboratory series is very effective in teaching students the theory and practice of protein purification and analysis while also demonstrating positive results in more broad areas of scientific skill and knowledge. Amongst the findings, the largest reported increases in knowledge were related to students' understanding of how patent law relates to laboratory science, a topic of great importance to modern researchers that is readily discussed in relation to Taq polymerase. Overall, this laboratory series proves to be a very effective component in the curricula of undergraduate biology and chemistry majors and may be an appropriate laboratory experience for undergraduates.