Sericin/RBA embedded gellan gum based smart nanosystem for pH responsive drug delivery
Sericin/RBA embedded gellan gum based smart nanosystem for pH responsive drug delivery
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DOI：
10.1016/j.ijbiomac.2018.09.146
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发表时间：
2018-12-01
影响因子：
8.2
通讯作者：
Jeyaraj, Murugaraj
中科院分区：
文献类型：
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作者：
Arjama, Mukherjee;Mehnath, Sivaraj;Jeyaraj, Murugaraj
多糖蛋白复合物在给药系统中为合成聚合物提供了一种绿色替代品。丝胶蛋白（SC）是一种天然蛋白，与米糠白蛋白（RBA）和结冷胶（GG）结合形成绿色蛋白多糖复合物。采用不同的表征技术对丝胶功能化结冷胶-米糠纳米复合材料进行了表征。这表明它们在平衡纳米载体的生物相容性、稳定性、生物降解性和功能性方面具有突出的能力。纳米复合材料呈球形，核心为蛋白-多糖结构，平均尺寸约为218 nm。采用SC-GG-RBA纳米复合材料包埋大量阿霉素（DOX），研究其在酸性肿瘤环境中的有效释放。在pH 4.0条件下120 h， 84%的DOX在体外释放。负载DOX的绿色纳米复合材料的IC50为5 μ g/mL，与MCF-7细胞处理后的自由DOX （9 μ g/mL）相比非常低。绿色纳米复合材料处理后，只有42%的细胞存活。这是由于癌细胞对纳米材料的有效吸收和DOX在细胞质区域的直接释放。这种高性能绿色纳米复合材料在扩大自然资源生物材料的利用和在生物医学领域的合理应用方面具有很大的潜力。(C) 2018 Elsevier B.V.版权所有
Polysaccharides protein complex offers a green alternative to synthetic polymers in the drug delivery system. Sericin (SC), a natural protein, in combination with rice bran albumin (RBA) and gellan gum (GG) forms a green based protein polysaccharide complex. The sericin functionalized gellan gum-rice bran (SC-GG-RBA) nano composites were characterized by different characterization techniques. It shows their prominent ability in balancing the biocompatibility, stability, biodegradability, and functionality of nanocarriers. The nanocomposites exhibited spherical shape with core protein-polysaccharide structures, and the average size was about 218 nm. High amount of Doxorubicin (DOX) was encapsulated into SC-GG-RBA nanocomposites in order to investigate the effective drug release in acidic tumor environment. DOX of 84% was released in vitro condition after 120 h in pH 4.0. DOX loaded green nanocomposites shows IC50 5 mu g/mL which was very low compared to free DOX of 9 mu/mL after treatment with MCF-7 cells. Only 42% of cells were survived after treatment with green nano composites. This was due to the effective uptake of nanomaterial by cancer cells and direct release of DOX in cytoplasmic region. Such high performance green nanocomposites have great potential in expanding the utilization of biomaterial from natural resources and development of sensible application in biomedical field. (C) 2018 Elsevier B.V. All rights reserved.