Uterine macrophages express the gp49B inhibitory receptor in midgestation
Uterine macrophages express the gp49B inhibitory receptor in midgestation
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DOI：
10.4049/jimmunol.166.2.781
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发表时间：
2001-01-15
影响因子：
4.4
通讯作者：
Aso, T
中科院分区：
文献类型：
--
作者：
Matsumoto, Y;Wang, LL;Aso, T
小鼠 gp49B 是一种在肥大细胞和 NK 细胞上发现的基于免疫受体酪氨酸的抑制性基序受体。然而，在本报告中，我们表明该受体在妊娠中期子宫内膜腺中积聚的巨噬细胞上表达，与 gp49A 一起表达，gp49A 具有与 gp49B 非常同源的细胞外结构域，但具有较短的细胞质结构域，而没有 ITIM。在子宫腺的条件培养基中培养骨髓细胞导致表达Fcγ激活受体和gp49B抑制性受体的巨噬细胞的选择性增殖。用固定化 IgG（而非抗 Fc γ RII/III）刺激巨噬细胞，诱导产生大量 TNF-α 和 IL-10，表明 IgG 的高亲和力受体（Fc γ RI）可以在巨噬细胞产生细胞因子时传递激活信号。此外，gp49B 与 Fc gamma RI 的连接导致 TNF-α 产生的抑制。因此，我们的数据提供证据表明 gp49B 是巨噬细胞激活的内源性负调节因子，并且可能调节怀孕期间巨噬细胞的功能。
Mouse gp49B is an immunoreceptor tyrosine-based inhibitory motif-bearing receptor identified on mast cells and NK cells. In this report, however, we show that this receptor is expressed on macrophages accumulating in the uterine metrial gland in midgestation, along with gp49A that has a very homologous extracellular domain with gp49B but has a short cytoplasmic domain without ITIM. Culture of bone marrow cells in the conditioned medium of the metrial gland resulted in the selective proliferation of macrophages expressing both Fc gamma -activating receptors and gp49B inhibitory receptor. Stimulation of macrophages with immobilized IgG, but not with anti-Fc gamma RII/III, induced a considerable amount of TNF-alpha and IL-10 production, suggesting that the high-affinity receptor for IgG (Fc gamma RI) can transmit activating signals in cytokine production of macrophages. Furthermore, coligation of gp49B with Fc gamma RI resulted in the inhibition of TNF-alpha production. Thus, our data provide evidence that gp49B is an endogenous negative regulator of macrophage activation and may regulate the function of macrophages during pregnancy.