Expression Profile of CYP1A1 and CYP1B1 Enzymes in Colon and Bladder Tumors
Expression Profile of CYP1A1 and CYP1B1 Enzymes in Colon and Bladder Tumors
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DOI：
10.1371/journal.pone.0082487
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发表时间：
2013-12-16
期刊：
影响因子：
3.7
通讯作者：
Tsatsakis, Aristidis M.
中科院分区：
文献类型：
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作者：
Androutsopoulos, Vasilis P.;Spyrou, Ioannis;Tsatsakis, Aristidis M.
背景：细胞色素P450、细胞色素P1A1、细胞色素P450、细胞色素1A1、细胞色素P450、细胞色素P450、细胞色素1A1、细胞色素P450、细胞色素P450、细胞色素QRT-PCR和免疫组织化学研究表明，在人类肿瘤中，细胞色素P1A1和细胞色素P1B1的水平升高。结果：本研究采用定量逆转录聚合酶链式反应(qRT-PCR)和酶活性分析法检测了一组人膀胱和结直肠肿瘤组织中细胞色素P4501A1和细胞色素P1B1的表达。结果显示，35%(7/20)的膀胱肿瘤和35%(7/20)的结肠肿瘤过度表达活性的CYP1酶。在膀胱癌和结肠癌中分别有65%和60%的肿瘤组织中有高表达，而在膀胱和结肠肿瘤中分别有65%和80%的肿瘤组织中有高表达。膀胱癌和结肠癌组织中细胞色素P450 1B1和细胞色素P1A1的平均mRNA水平和活性均高于正常组织(P
Background: The cytochrome P450 CYP1A1 and CYP1B1 enzymes are involved in carcinogenesis via activation of pro-carcinogenic compounds to carcinogenic metabolites. CYP1A1 and CYP1B1 have shown elevated levels in human tumors as determined by qRT-PCR and immunohistochemical studies. However studies that have examined CYP1 expression by enzyme activity assays are limited.Results: In the current study the expression of CYP1A1 and CYP1B1 was investigated in a panel of human tumors of bladder and colorectal origin by qRT-PCR and enzyme activity assays. The results demonstrated that 35% (7/20) of bladder tumors and 35% (7/20) of colon tumors overexpressed active CYP1 enzymes. CYP1B1 mRNA was overexpressed in 65% and 60% of bladder and colon tumors respectively, whereas CYP1A1 was overexpressed in 65% and 80% of bladder and colon tumors. Mean mRNA levels of CYP1B1 and CYP1A1 along with mean CYP1 activity were higher in bladder and colon tumors compared to normal tissues (p