PCR CLONING OF AN ORPHAN HOMEOBOX GENE (PRH) PREFERENTIALLY EXPRESSED IN MYELOID AND LIVER-CELLS
PCR CLONING OF AN ORPHAN HOMEOBOX GENE (PRH) PREFERENTIALLY EXPRESSED IN MYELOID AND LIVER-CELLS
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DOI：
10.1006/bbrc.1993.2140
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发表时间：
1993-09-15
影响因子：
3.1
通讯作者：
COLLINS, S
中科院分区：
文献类型：
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作者：
HROMAS, R;RADICH, J;COLLINS, S
同源框基因是转录因子家族的成员，在许多不同的生物体中调节组织发育。我们开始寻找可能在造血中起作用的同源异型盒基因。利用与聚合酶链式反应(PCR)中保守的同源盒序列相对应的简并寡核苷酸引物，我们从HL60早幼粒细胞系中克隆了一个同源盒基因，我们现在将其命名为PRH。我们将这个与之前描述的任何同源框基因都不同的基因定位到10号染色体上，这是已知的基因组同源框簇之外的一个区域。Northern印迹杂交结果表明，PRH主要在髓系细胞和肝细胞中表达。PRH的结构、染色体位置和表达模式表明，它是一个孤儿同源框基因，可能调节某些造血细胞的谱系发育。
Homeobox genes are members of a family of transcription factors that regulate tissue development in many different organisms. We set out to identify homeobox genes that might play a role in hematopoiesis. Using degenerate oligonucleotide primers corresponding to conserved homeobox sequences in the polymerase chain reaction (PCR), we cloned from the HL60 promyelocytic cell line a homeobox gene we now designate PRH. We mapped this gene, which was not homologous to any of the previously described homeobox genes, to chromosome 10, a region outside of the known genomic homeobox clusters. Northern blot hybridization indicates that PRH is preferentially expressed in myeloid and liver cells. The structure, chromosomal location, and pattern of expression of PRH indicate that it represents an orphan homeobox gene that may regulate the lineage development of certain hematopoietic cells.