Maternal exercise before and during pregnancy alleviates metabolic dysfunction associated with high-fat diet in pregnant mice, without significant changes in gut microbiota
Maternal exercise before and during pregnancy alleviates metabolic dysfunction associated with high-fat diet in pregnant mice, without significant changes in gut microbiota
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DOI：
10.1016/j.nutres.2019.08.002
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发表时间：
2019-09-01
影响因子：
4.5
通讯作者：
Umeda, Masataka
中科院分区：
文献类型：
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作者：
Chung, Eunhee;Grue, Katherine A.;Umeda, Masataka
尽管母亲在怀孕前和怀孕期间进行锻炼是有益的，但运动对怀孕期间微生物群变化的影响尚不清楚。在这里，我们测试了这样的假设：在高脂饮食 (HFD) 喂养的母鼠中，母鼠怀孕前和怀孕期间的运动会对葡萄糖稳态、胰腺细胞功能和肠道微生物群失调产生积极影响。雌性 C57BL/6 小鼠以 HFD 或低脂饮食 (LFD) 喂养 12 周。在妊娠开始前 4 周和整个妊娠期间，HFD 小鼠被分为两组：久坐（HFD）或运动（HFD + Ex）。测量食物摄入量、体重、身体成分、葡萄糖和胰岛素耐量。妊娠第 19 天时，收集血液、胰腺、性腺内脏和皮下脂肪、跖肌和盲肠进行分析。妊娠 15 天时，与 LFD 小鼠相比，HFD 和 HFD + Ex 小鼠的葡萄糖清除率均受损。未发现胰腺 a 细胞或 It 细胞健康状况发生变化。与 HFD 喂养的小鼠相比，HFD + Ex 小鼠的内脏脂肪量、血清胰岛素和瘦素水平显着降低，高密度脂蛋白水平增加。与我们的假设相反，不同群体之间的微生物群多样性和组成没有差异。五种细菌门的相对丰度，如纤毛菌门、拟杆菌门、疣微菌门、脱铁杆菌门和放线菌门，在怀孕期间的饮食或运动中没有显着改变。我们的研究结果表明，母亲运动可以防止与 HFD 相关的内脏脂肪过多堆积、高胰岛素血症和高瘦素血症，但不能通过怀孕期间肠道微生物群组成或多样性的改变来预防。 (C) 2019 Elsevier Inc. 保留所有权利。
Although maternal exercise before and during pregnancy is beneficial, the effects of exercise on microbiota changes during pregnancy are unknown. Here we tested the hypothesis that maternal exercise before and during pregnancy would positively affect glucose homeostasis, pancreatic cell function, and gut microbiota dysbiosis in high-fat diet (HFD) fed dams. Female C57BL/6 mice were fed either a HFD or a low-fat diet (LFD) for 12 weeks. The HFD mice were split into two groups for 4 weeks prior to pregnancy initiation and throughout the pregnancy: sedentary (HFD) or exercised (HFD + Ex). Food intake, body weight, body composition, and glucose and insulin tolerance were measured. At gestation day 19, blood, pancreas, gonadal visceral and subcutaneous fat, plantaris muscle, and cecum were collected for analysis. Both HFD and HFD + Ex mice had impaired glucose clearance compared to LFD mice at 15 days of gestation. No changes were found in pancreatic a- or It -cell health. HFD + Ex mice had significantly reduced visceral fat mass, serum insulin, and leptin levels and increased high-density lipoprotein levels, compared to HFD-fed mice. In contrast to our hypothesis, microbiota diversity and composition were not different among groups. The relative abundance of five bacterial phyla, such as Firrnicutes, Bacteroidetes, Verrucomicrobia, Deferribacteres, and Actinobacteria, were not significantly altered with diet or exercise during pregnancy. Our findings suggest that maternal exercise prevents excess visceral fat accumulation, hyperinsulinemia, and hyperleptinemia associated with a HFD, but not through the alterations of gut microbiota composition or diversity during pregnancy. (C) 2019 Elsevier Inc. All rights reserved.