IDENTIFICATION AND LOCALIZATION OF A NOVEL, CYTOSKELETAL, CENTROSOME-ASSOCIATED PROTEIN IN PTK2 CELLS
IDENTIFICATION AND LOCALIZATION OF A NOVEL, CYTOSKELETAL, CENTROSOME-ASSOCIATED PROTEIN IN PTK2 CELLS
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DOI：
10.1083/jcb.107.6.2669
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发表时间：
1988-12-01
影响因子：
7.8
通讯作者：
SALISBURY, JL
中科院分区：
文献类型：
--
作者：
BARON, AT;SALISBURY, JL
抗Centin(索尔兹伯里，J.L.，A.T.巴伦，B.Surek和M.Melkonian)。1984年。J.细胞生物学。99：962-970)已用于鉴定MR为165,000的中心体相关蛋白。免疫细胞化学表明，该蛋白是间期细胞的中央周围卫星、基底足和中央周围基质的组成部分。这些中心周围物质的成分部分由3-8 nm-直径的细丝组成，这些细丝相互连接形成三维的中心周围点阵。我们得出结论，165,000-MR蛋白在免疫上与Centin相关，并且它是一个新的中心体相关细胞骨架细丝系统的组成部分。微管组织中心，如藻类细胞的鞭毛器、酵母细胞的纺锤体和哺乳动物细胞的中心体，是细胞质组织和细胞增殖所必需的同源结构。最近的分子克隆研究表明，纺锤体极体复制所需的细胞周期基因产物CDC31与Centin(Huang，B.，A.Mengersen和V.D.Lee)有50%的序列同源性。1988年。J.细胞生物学。107：133-140)。中心素相关序列和免疫表位对不同系统发育的微管组织中心的进化保守表明，一个功能属性(S)可能也是保守的。阐明这些相关分子之间的共同线索可能是我们理解细胞结构和功能的基础。
Antisera raised against centrin (Salisbury, J. L., A. T. Baron, B. Surek, and M. Melkonian. 1984. J. Cell Biol. 99:962-970) have been used, here, to identify a centrosome-associated protein with an Mr of 165,000. Immunocytochemistry indicates that this protein is a component of pericentriolar satellites, basal feet, and pericentriolar matrix of interphase cells. These components of pericentriolar material are, in part, composed of 3-8-nm-diam filaments, which interconnect to form a three-dimensional pericentriolar lattice. We conclude that the 165,000-Mr protein is immunologically related to centrin, and that it is a component of a novel centrosome-associated cytoskeletal filament system. Microtubule organizing centers such as the flagellar apparatus of algal cells, spindle pole body of yeast cells, and centrosome of mammalian cells are homologous structures essential for cytoplasmic organization and cellular proliferation. Molecular cloning studies have recently shown that the cell cycle gene product CDC31, required for spindle pole body duplication, shares 50% sequence homology with centrin (Huang, B., A. Mengersen, and V. D. Lee. 1988. J. Cell Biol. 107:133-140). The evolutionary conservation of centrin-related sequences and immunologic epitopes to microtubule organizing centers of divergent phylogeny suggests that a functional attribute(s) may have been conserved as well. Elucidation of a common thread between these related molecules may be fundamental to our understanding of cell structure and function.