Circulating tumor-derived DNA may permit the early diagnosis of head and neck squamous cell carcinomas
Circulating tumor-derived DNA may permit the early diagnosis of head and neck squamous cell carcinomas
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DOI：
10.1002/1097-0215(200102)9999:9999
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发表时间：
2001-04-15
影响因子：
6.4
通讯作者：
Simpson, AJG
中科院分区：
文献类型：
--
作者：
Nunes, DN;Kowalski, LP;Simpson, AJG
应用8个微卫星位点对91例头颈部鳞状细胞癌进行了杂合性缺失和新突变等位基因的检测，其中58例检测到了变异，并将其作为检测患者血浆中循环肿瘤源DNA存在的标记。在17个案例中明确发现了这一点。检测循环DNA的概率是独立的肿瘤分期，并发现存在，甚至在一些个人与I期肿瘤。然而，这种DNA的存在与疾病结果或其他重要的临床参数无关，表明它没有预后意义。结果表明，循环肿瘤DNA可作为头颈部肿瘤早期诊断的一种手段。(C)2001 Wiley-Liss，Inc.
A series of eight microsatellite loci were assayed for both loss of heterozygosity and new mutated alleles in 91 head and neck squamous cell carcinomas, In 58 cases, alterations were detected and used as markers for assaying the presence of circulating tumor-derived DNA in the patients' plasma. This was unambiguously detected in 17 cases. The probability of detecting circulating DNA was independent of tumor stage and was found to be present even in some individuals with stage I tumors. The presence of such DNA, however, could not be correlated with disease outcome or other significant clinical parameters, suggesting that it has no prognostic significance. The results indicate that circulating tumor-derived DNA could be used as a means of early diagnosis of head and neck tumors. (C) 2001 Wiley-Liss, Inc.