Metal-enhanced fluorescence of an organic fluorophore using gold particles
Metal-enhanced fluorescence of an organic fluorophore using gold particles
复制标题
DOI：
10.1364/oe.15.002598
复制
发表时间：
2007-03-05
期刊：
影响因子：
3.8
通讯作者：
Lakowicz, Joseph R.
中科院分区：
文献类型：
--
作者：
Zhang, Jian;Lakowicz, Joseph R.
通过气相沉积将颗粒金薄膜沉积在玻璃基板上。通过物理吸附固定兔免疫球蛋白 G (IgG)，然后将 Alexa Fluor 抗兔 IgG 结合到蛋白质包被的表面。当使用 Alexa Fluor555 抗 IgG 时，荧光随着 Au 厚度的增加而增强，并在 30 nm 处达到饱和。我们还检查了金膜和标记蛋白质之间二氧化硅间隔物的影响。最大增强取决于二氧化硅的厚度，并在 10 nm 处达到最大值。强度最大增加约6倍。我们还将 Alexa Fluor-680 抗 IgG 结合到蛋白质包被的表面，最大增强约为 10 倍。 (c) 2007 年美国光学学会。
Particulate gold films were deposited on glass substrates by vapor deposition. Rabbit immunoglobulin G (IgG) was immobilized by physiosorption and then Alexa Fluor anti Rabbit IgG was bound to the protein-coated surfaces. Fluorescence was enhanced with increasing the Au thickness and reached saturation at 30 nm when Alexa Fluor555 anti IgG was used. We also examined the effect of silica spacers between the gold film and the labeled protein. The maximum enhancement was dependent on the thickness of silica and reach maximum at 10 nm. The maximum increase in intensity was about 6-fold. We also bound Alexa Fluor-680 anti IgG to the protein-coated surface, and the maximum enhancement was about 10-fold. (c) 2007 Optical Society of America.