TRANSPOSON MUTAGENESIS AS A TOOL TO STUDY THE ROLE OF HEMOLYSIN IN THE VIRULENCE OF LISTERIA-MONOCYTOGENES
TRANSPOSON MUTAGENESIS AS A TOOL TO STUDY THE ROLE OF HEMOLYSIN IN THE VIRULENCE OF LISTERIA-MONOCYTOGENES
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DOI：
10.1128/iai.52.1.50-55.1986
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发表时间：
1986-04-01
影响因子：
3.1
通讯作者：
SANSONETTI, P
中科院分区：
文献类型：
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作者：
GAILLARD, JL;BERCHE, P;SANSONETTI, P
通过转座子诱变研究了溶血素在单核细胞增生李斯特菌毒力中的作用。最初在肺炎链球菌中发现的 26 KB 接合转座子 Tn1545 被转移到单核细胞增生李斯特菌溶血性强毒菌株中。据估计，每个接收者的转移频率约为 10-8 次。这使我们能够分离出一种非溶血性突变体，该突变体很可能含有 Tn1545 的单个副本。溶血素产生的丧失与毒力的丧失相关。静脉内攻击后，突变体的 50% 致死剂量被评估为每只小鼠约 109.6 个细菌。非溶血性细菌无法在宿主组织中生长，并迅速从受感染小鼠的脾脏和肝脏中消除。通过转座子 Tn1545 自发丧失而获得的产生溶血素的回复体的毒力得以恢复。这些结果强烈表明溶血素是与单核细胞增生利斯特氏菌细胞内生长有关的主要毒力因子。
The role of hemolysin in the virulence of Listeria monocytogenes was studied by using transposon mutagenesis. The 26-kilobase conjugative transposon Tn1545, originally found in Streptococcus pneumoniae, was transferred to a hemolytic virulent strain of L. monocytogenes. The frequency of transfer was estimated to be about 10-8 per recipient. This allowed us to isolate a nonhemolytic mutant which most likely harbors a single copy of Tn1545. Loss of hemolysin production was associated with loss of virulence. The 50% lethal dose of the mutant was assessed to about 109.6 bacteria per mouse after intravenous challenge. Nonhemolytic bacteria were unable to grow in host tissues and were rapidly eliminated from the spleen and liver of infected mice. Virulence was restored in hemolysin-producing revertant obtained by spontaneous loss of transposon Tn1545. These results strongly suggest that hemolysin is a major virulence factor implicated in the intracellular growth of L. monocytogenes.